phospho-JNK1 (Thr183)一抗

Mouse Anti-phospho-JNK1 (Thr183)一抗 antibody

From:RICKY

Catalog Number : RS-97591R

Quantity size : 0.1ml (dilute with pH7.4 0.01 M PBS or antibody diluent )

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP

kinases act as an integration point for multiple biochemical signals, and are involved in a wide

variety of cellular processes such as proliferation, differentiation, transcription regulation and

development. This kinase is activated by various cell stimuli, and targets specific transcription

factors, and thus mediates immediate-early gene expression in response to cell stimuli. The

activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for

TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis,

which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse

counterpart of this gene suggested that this kinase play a key role in T cell proliferation,

apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct

isoforms have been reported. [provided by RefSeq, Jun 2013]

Function:

Responds to activation by environmental stress and pro-inflammatory cytokines by

phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN,

JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are

required for polarized differentiation of T-helper cells into Th1 cells (By similarity).

Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding

patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a simphospho-JNK1 (Thr183)一抗ilar

low level of binding to both c-Jun or ATF2. However, there is no correlation between binding

and phosphorylation, which is achieved at about the same efficiency by all isoforms.

pecificity :

– Anti-phospho-JNK1 (Thr183) is a mouse monoclonal antibody

– specific for phospho-JNK1 (Thr183) of rabbit

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

– Protein A affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 50 kDa

Application :

– Western blotting 1:100-500

– Immunohistochemistry 1:100-500

– ELISA 1:500-1000

– Optimal working dilutions must be determined by the end user.

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The

lyophilized antibody is stable at room temperature for at least one month and for

greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M

PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for

use in human, therapeutic or diagnostic applications.

phospho-JNK1 (Thr183)一抗