α1-微球蛋白(α1M)多克隆抗体

α1-微球蛋白(α1M)多克隆抗体 

Polyclonal Antibody to Alpha-1-Microglobulin/Bikunin Precursor (a1M)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species    Mus musculus (Mouse)    

Product No.    YB217Mu02    

Clonality    Polyclonal    

Host    Rabbit    

Immunoglobulin Type    IgG    

Purification    Affinity Chromatography    

Applications    WB, ICC, IHC-P, IHC-F, ELISA    

Concentration    200ug/ml    

UOM    100ug    

Conjugate    No Conjugate    

ANTIBODY SPECIFITY

The antibody is a rabbit polyclonal antibody raised against a1M. It has been selected for its ability to recognize a1M in immunohistochemical staining and western blotting.

APPLICATIONS

Western blotting: 1:50-400 
Immunocytochemistry in formalin fixed cells: 1:50-500 
Immunohistochemistry in formalin fixed frozen section: 1:50-500 
Immunohistochemistry in paraffin section: 1:10-100 
Enzyme-linked Immunosorbent Assay: 1:100-200 
Optimal working dilutions must be determined by end user. 

CONTENTS

Form & Buffer: Supplied as solution form in PBS, pH7.4, 
containing 0.02% NaN3, 50% glycerol.

IHC Images

QUALITY CONTROL

Content: The quality control contains recombinant a1M (Arg18~Ala202) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

STORAGE

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.