髓鞘碱性蛋白(MBP)多克隆抗体

上海钰博生物科技有限公司

2016/01/05 14:46

TA的动态

髓鞘碱性蛋白(MBP)多克隆抗体

Polyclonal Antibody to Myelin Basic Protein (MBP)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species Homo sapiens (Human)

Product No. ybA539Hu01

Clonality Polyclonal

Host Rabbit

Immunoglobulin Type IgG

Purification Affinity Chromatography

Applications WB, ICC, IHC-P, IHC-F, ELISA

Concentration 200ug/ml

UOM 100ug

Conjugate No Conjugate

髓鞘碱性蛋白(MBP)多克隆抗体ANTIBODY SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MBP. It has been selected for its ability to recognize MBP in immunohistochemical staining and western blotting.

APPLICATIONS

Western blotting: 1:50-400
髓鞘碱性蛋白(MBP)多克隆抗体Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

CONTENTS

Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.

IHC Images

髓鞘碱性蛋白(MBP)多克隆抗体

Used in DAB staining on fromalin fixed paraffin-embedded Brain tissue

QUALITY CONTROL

Content: The quality control contains recombinant MBP (Val220~Met301) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

STORAGE

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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