自动pH滴定期间肌红蛋白结构的CD检测

2019/12/22   下载量： 1 次

方案摘要

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应用领域 生物产业
检测样本 其他
检测项目
参考标准 无

Circular dichroism spectroscopy (CD) is very sensitive to the secondary structure of polypeptides and proteins. Circular dichroism is a form of light absorption spectroscopy that measures the difference in absorbance of rightand left-circularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. It has been shown that CD spectra between 260 and approximately 180 nm can be analyzed for the different secondary structure types: alpha helix, beta sheet, beta turn, random coils, etc. Secondary structure determination by CD is reported to achieve accuracies of 0.97 for helices, 0.75 for beta sheet, 0.50 for turns, and 0.89 for other structure types. In this application CD will be used to follow the denaturation of myoglobin during an automated pH titration with acid.

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Circular dichroism spectroscopy (CD) is very  sensitive to the secondary structure of  polypeptides and proteins. Circular dichroism is a  form of light absorption spectroscopy that  measures the difference in absorbance of rightand left-circularly polarized light (rather than the  commonly used absorbance of isotropic light) by a  substance. It has been shown that CD spectra  between 260 and approximately 180 nm can be  analyzed for the different secondary structure  types: alpha helix, beta sheet, beta turn, random  coils, etc. Secondary structure determination by  CD is reported to achieve accuracies of 0.97 for  helices, 0.75 for beta sheet, 0.50 for turns, and 0.89  for other structure types. In this application CD  will be used to follow the denaturation of  myoglobin during an automated pH titration with  acid.

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