超高速视频级原子力显微镜—HS-AFM
超高速视频级原子力显微镜—HS-AFM
超高速视频级原子力显微镜—HS-AFM
优选

超高速视频级原子力显微镜—HS-AFM

参考价:面议
型号: HS-AFM
产地: 日本
品牌: RIBM
评分:
应用领域:
核心参数
关注展位 全部仪器
展位推荐 更多
产品详情

超高速视频级原子力显微镜—HS-AFM

 

原子力显微镜(Atomic Force Microscope,AFM),一种可用来研究包括绝缘体在内的固体材料表面结构的分析仪器。它通过检测待测样品表面和一个微型力敏感元件之间的极微弱的原子间相互作用力来研究物质的表面结构及性质。将一对微弱力极端敏感的微悬臂一端固定,另一端的微小针尖接近样品,这时它将与其相互作用,作用力将使得微悬臂发生形变或运动状态发生变化。扫描样品时,利用传感器检测这些变化,就可获得作用力分布信息,从而以纳米级分辨率获得表面形貌结构信息及表面粗糙度信息。原子力显微镜可以测量材料物理性质、力学性能、磁学性能、热学性能、电学性能等方面的一些特征信息,但在扫描成像速度上一直存在局限性,太慢的扫描速度导致原子力显微镜无法捕捉到分子间的相互作用过程和一些快速的分子动态变化。

 

超高速视频级原子力显微镜(High-Speed Atomic Force Microscope,HS-AFM)由日本 Kanazawa 大学 Prof. Ando 教授团队研发,日本RIBM公司(生体分子计测研究所株式会社,Research Institute of Biomolecule Metrology Co., Ltd)商业化的产品,可以达到视频级成像的商业化原子力显微镜。HS-AFM突破了传统原子力显微镜“扫描成像速慢”的限制,能够在液体环境下超快速动态成像,分辨率为纳米水平。样品无需特殊固定,不影响生物分子的活性,尤其适用于生物大分子互作动态观测。超高速视频级原子力显微镜HS-AFM主要有两种型号,SS-NEX样品扫描(Sample-Scanning HS-AFM)以及PS-NEX探针扫描(Probe-Scanning HS-AFM)。推出至今,全球已有100多位用户,发表 SCI 文章 300 余篇,包括Science, Nature, Cell 等顶级杂志。

 

相较于目前市场上的原子力显微镜成像设备,HS-AFM突破了 “扫描成像速慢”的限制,扫描速度高可达 20 frame/s,并且有 4 种扫描台可供选择。样品无需特殊固定染色,不影响生物分子的活性,尤其适用于生物大分子互作动态观测。液体环境下直接检测,超快速动态成像,分辨率为纳米水平。探针小,适用于生物样品;悬臂探针共振频率高,弹簧系数小,避免了对生物样品等的损伤。悬臂探针可自动漂移校准,适用于长时间观测。采用动态PID控制,高速扫描时仍可获得清晰的图像。XY轴分辨率2nm;Z轴分辨率0.5nm。

HS-AFM不仅拥有超高扫描速率与原子级别分辨率,而且具有操作的简易性,使得对单分子动态过程的捕捉变得十分方便,为科研工作者研所和理解生物物理、生物化学、分子生物学、病毒学以及生物医学等领域的单分子动态过程提供了一款强大的工具。

全新的HS-AFM采用了新的高频微悬臂架构,更低噪音、更高稳定性的2控制器,高速扫描器,缓冲防震设计,主动阻尼,动态PID,驱动算法优化,多种前沿技术,可以实现在超高速下获取高分辨的生物样品信息。新系统整合了基于工作流程的操作软件,直观的用户界面与流程化、自动化的设置使得研究人员可以专注于实验设计,不需要复杂的操作和条件设置,快速获取数据,加速研究的产出。

 


日本RIBM公司的超高速视频级原子力显微镜HS-AFM的创新点:

★   高频微悬臂

弹性系数:   0.1 N/m   

曲率半径:   <10 nm 

共振频率:   400-600kHz in liquid

★   高速扫描台

20 frames/s.   (Standard scanner)

★   缓冲防震+主动阻尼+动态PID+算法优化

缓冲防震

主动阻尼

动态PID控制:可自动改变反馈增益,保证了HS-AFM在高速扫描条件下仍可获得清晰的图像

探针自动漂移校准,适用于长时间样品观测

 

     

 

日本RIBM公司的超高速视频级原子力显微镜HS-AFM的的应用领域:

 

从单分子到单细胞,都可直接观测

 

 

1、肌动蛋白

 

2、CRISPR-Cas9

 

 

3、膜联蛋白

 

 

4、IgG

 

 

5、活细胞

 

 

6、细菌视紫红质

 

 

7、DNA纳米结构

 

 

8、仿生聚合物

 

 

日本RIBM公司的超高速视频级原子力显微镜HS-AFM的视频案例

 

1:IgG

在溶液中观察到抗体(IgG)。

IgG呈"Y"形,两个Fab区区分清晰。

由于锚定能力较弱,IgG保持其抗原结合能力。

 

 

 

2:Plasmid DNA

传统AFM在没有强锚定的情况下,DNA分子图像出现摆动。

然而,强锚定可能会削弱真实的结构和行为。

HS-AFM能清晰显示质粒的结构和运动,无强锚定。

 

 

 

3:DNA内切酶的消化:DNase I

DNA酶I是一种随机消化DNA的核酸内切酶。视频中的箭头表示DNase I消化DNA的部分。

请参考从DNA末端消化的核酸外切酶Bal31的视频。

 

 

 

4:DNA外切酶消化:Bal31

Bal31是一种从DNA链末端消化DNA的核酸外切酶。

视频显示Bal31的活性沿着DNA移动,并逐渐从DNA链的末端消化。

最后,DNA分子被完全消化,但环状DNA未被消化。高光点是Bal31分子,它们与DNA的不同位置结合。
 

 

 

5:DNA聚合酶的DNA延伸:Phi29

双链DNA(黄色)随着时间的推移而拉长。单链λDNA作为模具固定在基板上。

由于从随机六聚体引物(Red)结合到λDNA模体,phi29聚合酶(Black)以dNTP为底物合成互补DNA。
 

 

 

6:链亲和素2D晶体中的点缺陷

成功地观察到点缺陷在晶体中的扩散。

从图像上看,两个单空位缺陷的轨迹跟踪相对于晶格的两个轴是明显的各向异性的。

 

 

 

 

日本RIBM公司的超高速视频级原子力显微镜HS-AFM的文献列表

 

Title

Journal

Biophysical reviews top five: atomic force microscopy in biophysics

Biophysical Reviews

Reconstruction of Three-Dimensional Conformations of Bacterial
ClpB from High-Speed Atomic-Force-Microscopy Images

Frontiers in Molecular
Biosciences

A facile combinatorial approach to construct a ratiometric fluorescent
sensor: application for the real-time sensing of cellular pH changes

Chemical Science

DNA Nanotechnology to Disclose Molecular Events at the Nanoscale
and Mesoscale Levels

Springer Nature

Quantitative description of a contractile macromolecular machine

Science Advances

Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ
Visualized by High-Speed Atomic Force Microscopy

International Journal of
Molecular Sciences 2021, Vol. 22, Page 1697

Localization atomic force microscopy

Nature 2021 594:7863

Movements of mycoplasma mobile gliding machinery detected by
high-speed atomic force microscopy

mBio

An ultra-wide scanner for large-area high-speed atomic force
microscopy with megapixel resolution

Scientific Reports 2021 11:1

A molecularly engineered, broad-spectrum anti-coronavirus lectin
inhibits SARS-CoV-2 and MERS-CoV infection in vivo

Research Square

Influenza virus ribonucleoprotein complex formation occurs in the
nucleolus

bioRxiv

Tardigrade Secretory-Abundant Heat-Soluble Protein Has a Flexible
β-Barrel Structure in Solution and Keeps This Structure in
Dehydration

Journal of Physical Chemistry B

Ultrastructure of influenza virus ribonucleoprotein complexes during
viral RNA synthesis

Communications Biology on

A facile combinatorial approach to construct a ratiometric fluorescent
sensor: application for the real-time sensing of cellular pH changes

Chemical Science

Deformation of microtubules regulates translocation dynamics of
kinesin

Science Advances

Unraveling the host-selective toxic interaction of cassiicolin with lipid
membranes and its cytotoxicity

bioRxiv

Nanostructure and thermoresponsiveness of poly( N -isopropyl
methacrylamide)-based hydrogel microspheres prepared via aqueous free radical precipitation polymerization

RSC Advances

JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form
tubular recycling endosomes

Communications Biology 2021
4:1

Correlation of membrane protein conformational and functional
dynamics

Nature Communications 2021
12:1

Non-close-packed arrangement of soft elastomer microspheres on
solid substrates

RSC Advances

Folding RNA–Protein Complex into Designed Nanostructures

Methods in Molecular Biology

A glutamine sensor that directly activates TORC1

Communications Biology 2021
4:1

Architecture of zero-latency ultrafast amplitude detector for high-
speed atomic force microscopy

Applied Physics Letters

Correlative AFM and fluorescence imaging demonstrate nanoscale
membrane remodeling and ring-like and tubular structure formation by septins

Nanoscale

Desiccation-induced fibrous condensation of CAHS protein from an
anhydrobiotic tardigrade

bioRxiv

Construction of ferritin hydrogels utilizing subunit–subunit
interactions

PLOS ONE

Monomeric α-synuclein (αS) inhibits amyloidogenesis of human
prion protein (hPrP) by forming a stable αS-hPrP hetero-dimer.

Prion

Influence of protein adsorption on aggregation in prefilled syringes

Journal of Pharmaceutical
Sciences

Dynamic mechanisms of CRISPR interference by Escherichia coli
CRISPR-Cas3

bioRxiv

An RNA Triangle with Six Ribozyme Units Can Promote a Trans-
Splicing Reaction through Trimerization of Unit Ribozyme Dimers

Applied Sciences

Faster high-speed atomic force microscopy for imaging of
biomolecular processes

Rev. Sci. Instrum

Title: Identification of lectin receptors for conserved SARS-CoV-2
glycosylation sites

bioRxiv

Structural and dynamics analysis of intrinsically disordered proteins
by high-speed atomic force microscopy

Nature Nanotechnology

Millisecond Conformational Dynamics of Skeletal Myosin II Power
Stroke Studied by High-Speed Atomic Force Microscopy

ACS Nano

High-Speed Atomic Force Microscopy Reveals Spatiotemporal
Dynamics of Histone Protein H2A Involution by DNA Inchworming

The Journal of Physical
Chemistry Letters

Nanostructures, Thermoresponsiveness, and Assembly Mechanism
of Hydrogel Microspheres during Aqueous Free-Radical Precipitation Polymerization

Langmuir

Chained structure of dimeric F 1-like ATPase in Mycoplasma mobile
gliding machinery 4

bioRxiv

Lipid Membrane Interaction of Peptide/DNA Complexes Designed for
Gene Delivery

Langmuir

High-Speed Atomic Force Microscopy to Study Myosin Motility

Advances in Experimental
Medicine and Biology

Single-molecule level dynamic observation of disassembly of the
apo-ferritin cage in solution

Physical Chemistry Chemical
Physics

Atomic Force Microscopy of Biomembranes : A Tool for Studying the
Dynamic Behavior of Membrane Proteins

New Techniques for Studying
Biomembranes

Adenosine leakage from perforin-burst extracellular vesicles inhibits
perforin secretion by cytotoxic T-lymphocytes

PLOS ONE

High-Speed Atomic Force Microscopy Reveals the Structural
Dynamics of the Amyloid-β and Amylin Aggregation Pathways

International Journal of
Molecular Sciences 2020, Vol. 21, Page 4287

Molecular mechanism of the recognition of bacterially cleaved
immunoglobulin by the immune regulatory receptor LILRA2

Journal of Biological Chemistry

Biological physics by high-speed atomic force microscopy

Philosophical Transactions of
the Royal Society A: Mathematical, Physical and Engineering Sciences

Viral RNA recognition by LGP2 and MDA5, and activation of signaling
through step-by-step conformational changes

Nucleic Acids Research

Key Nucleation Stages and Associated Molecular Determinants and
Processes in pH-Induced Formation of Amyloid Beta Oligomers as Revealed by High-Speed AFM

bioRxiv

Novel Babesia bovis exported proteins that modify properties of
infected red blood cells

PLoS Pathogens

DNA origami demonstrate the unique stimulatory power of single
pMHCs as T-cell antigens

Proceedings of the National
Academy of Sciences

Structural insights into the mechanism of rhodopsin
phosphodiesterase

Nature Communications

Structural insights into the mechanism of rhodopsin
phosphodiesterase

Nature Communications

High-Speed AFM Reveals Molecular Dynamics of Human Influenza A
Hemagglutinin and Its Interaction with Exosomes

Nano Letters

DNA Ring Motif with Flexible Joints

Micromachines

Nanopores: a versatile tool to study protein dynamics

Essays in Biochemistry

Geometrical Characterization of Glass Nanopipettes with Sub-10 nm
Pore Diameter by Transmission Electron Microscopy

Analytical Chemistry

Carbon nanotube porin diffusion in mixed composition supported
lipid bilayers

Scientific Reports

One-Step Calibration of AFM in Liquid

Frontiers in Physics

Nanoscale interaction of RecG with mobile fork DNA

Nanoscale Advances

Convergent evolution of processivity in bacterial and fungal
cellulases

Proceedings of the National
Academy of Sciences

Convergent evolution of processivity in bacterial and fungal
cellulases

Proceedings of the National
Academy of Sciences of the United States of America

Interaction of the motor protein SecA and the bacterial protein
translocation channel SecYEG in the absence of ATP

Nanoscale Advances

Nanoreporter of an Enzymatic Suicide Inactivation Pathway

Nano Letters

High-speed atomic force microscopy highlights new molecular
mechanism of daptomycin action

Nature Communications

Direct visualization of the conformational change of FUS/TLS upon
binding to promoter-associated non-coding RNA

Chemical Communications

Thermoresponsive Micellar Assembly Constructed from a Hexameric
Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System

Journal of the American
Chemical Society

Schizorhodopsins: A family of rhodopsins from Asgard archaea that
function as light-driven inward H + pumps

Science Advances

Two-State Exchange Dynamics in Membrane-Embedded
Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM

Journal of Molecular Biology

Thermoresponsive structural changes of single poly(N-isopropyl
acrylamide) hydrogel microspheres under densely packed conditions on a solid substrate

Polymer Journal

High-Speed Atomic Force Microscopy Reveals Factors Affecting the
Processivity of Chitinases during Interfacial Enzymatic Hydrolysis of Crystalline Chitin

ACS Catalysis

Rad50 zinc hook functions as a constitutive dimerization module
interchangeable with SMC hinge

Nature Communications

Assembly mechanism of a supramolecular MS-ring complex to
initiate bacterial flagellar biogenesis in vibrio species

Journal of Bacteriology

Structural Dynamics of a Protein Domain Relevant to the Water-
Oxidizing Complex in Photosystem II as Visualized by High-Speed Atomic Force Microscopy

Journal of Physical Chemistry B

Recent advances in bioimaging with high-speed atomic force
microscopy

Biophysical Reviews

Dynamic behavior of an artificial protein needle contacting a
membrane observed by high-speed atomic force microscopy

Nanoscale

Enhanced enzymatic activity exerted by a packed assembly of a
single type of enzyme

Chemical Science

High Speed AFM and NanoInfrared Spectroscopy Investigation of A
β1–42 Peptide Variants and Their Interaction With
POPC/SM/Chol/GM1 Model Membranes

Frontiers in Molecular
Biosciences

The hierarchical assembly of septins revealed by high-speed AFM

Nature Communications

Millisecond dynamics of an unlabeled amino acid transporter

Nature Communications

Atg9 is a lipid scramblase that mediates autophagosomal membrane
expansion

Nature Structural & Molecular
Biology

A Simplified Cluster Analysis of Electron Track Structure for
Estimating Complex DNA Damage Yields

International Journal of
Molecular Sciences

Supramolecular tholos-like architecture constituted by archaeal
proteins without functional annotation

Scientific Reports

Studies on the impellers generating force in muscle

Biophysical Reviews

Diversity of physical properties of bacterial extracellular membrane
vesicles revealed through atomic force microscopy phase imaging

Nanoscale

High-Speed AFM Reveals Molecular Dynamics of Human Influenza A
Hemagglutinin and Its Interaction with Exosomes

Nano Letters

DNA density-dependent uptake of DNA origami-based two-or three-
dimensional nanostructures by immune cells

Nanoscale

Spatiotemporally tracking of nano-biofilaments inside the nuclear
pore complex core

Biomaterials

High-speed atomic force microscopy directly visualizes
conformational dynamics of the HIV Vif protein in complex with three host proteins

Journal of Biological Chemistry

Self- and Cross-Seeding on α-Synuclein Fibril Growth Kinetics and
Structure Observed by High-Speed Atomic Force Microscopy

ACS Nano

Liquidity Is a Critical Determinant for Selective Autophagy of Protein
Condensates

Molecular Cell

Capturing transient antibody conformations with DNA origami
epitopes

Nature Communications

Biophysics in Kanazawa University

Biophysical Reviews

Dynamics of oligomer and amyloid fibril formation by yeast prion
Sup35 observed by high-speed atomic force microscopy

Proceedings of the National
Academy of Sciences

Annexin-V stabilizes membrane defects by inducing lipid phase
transition

Nature Communications

Structure and mechanism of bactericidal mammalian perforin-2, an
ancient agent of innate immunity

Science Advances

Atomic Force Microscopy Visualizes Mobility of Photosynthetic
Proteins in Grana Thylakoid Membranes

Biophysical Journal

Construction of a Hexameric Hemoprotein Sheet and Direct
Observation of Dynamic Processes of Its Formation

Chemistry Letters

Single-molecule imaging analysis reveals the mechanism of a high-
catalytic-activity mutant of chitinase A from Serratia marcescens

Journal of Biological Chemistry

Direct observation and analysis of TET-mediated oxidation
processes in a DNA origami nanochip

Nucleic Acids Research

On-membrane dynamic interplay between anti-GM1 IgG antibodies
and complement component C1q

International Journal of
Molecular Sciences

Zwitterionic Polypeptides: Chemoenzymatic Synthesis and Loosening
Function for Cellulose Crystals

Biomacromolecules

 

 


北京佰司特科技有限责任公司为您提供RIBM超高速视频级原子力显微镜—HS-AFM,RIBMHS-AFM产地为日本,属于进口扫描探针显微镜SPM(原子力显微镜AFM、扫描隧道显微镜STM),除了超高速视频级原子力显微镜—HS-AFM的参数、价格、型号、原理等信息外,还可为您提供多功能蛋白稳定性分析仪—PSA-16,佰司特客服电话400-860-5168转4551,售前、售后均可联系。

行业应用
典型用户
用户单位 采购时间
同济大学 2023/04/07
大连理工大学 2023/02/16
浙江大学 2022/12/01
相关仪器 更多
相关资料 更多

相关产品

北京佰司特科技有限责任公司为您提供RIBM超高速视频级原子力显微镜—HS-AFM,RIBMHS-AFM产地为日本,属于进口扫描探针显微镜SPM(原子力显微镜AFM、扫描隧道显微镜STM),除了超高速视频级原子力显微镜—HS-AFM的参数、价格、型号、原理等信息外,还可为您提供多功能蛋白稳定性分析仪—PSA-16,佰司特客服电话400-860-5168转4551,售前、售后均可联系。
Business information
工商信息 信息已认证
当前位置: 佰司特 仪器 超高速视频级原子力显微镜—HS-AFM

关注

拨打电话

留言咨询