IGFBP-3 蛋白，人类，重组（His 标签）

IGFBP-3 Protein, Human, Recombinant (His Tag)

别称：BP-53 Protein, Human; IBP3 Protein, Human; IGFBP3 Protein, Human

纯度:> 85 % as determined by SDS-PAGE

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method

生物活性：1. Measured by its binding ability in a functional ELISA. Immobilized human IGF2 (Cat:13032-H24E) at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 18 ng/mL.

2. Measured by its binding ability in a functional ELISA. Immobilized human IGF1 (Cat:10598-H24E) at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 24 ng/mL.

3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.05-0.2 μg/mL in the presence of 14 ng/mL human IGFII.

蛋白构建：A DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2) (Gly28-Lys291) was fused with a polyhistidine tag at the N-terminus.

NP No.：NP_000589.2

表达宿主：HEK293 Cells

种属：Human

预测 N 端：His

分子量：The secreted recombinant human IGFBP3 comprises 280 amino acids with a predicted molecular mass of 31 kDa. As a result of glycosylation, the apparent molecular mass of rhIGFBP3 is approximately 40-45 kDa in SDS-PAGE under reducing conditions.

缓冲液：Lyophilized from sterile PBS, pH 7.4.

Please contact us for any concerns or special requirements.

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

运输方式：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件：Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶：A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.