上海科技大学--沈庆涛-- Self-capping of nucleoprotein filaments protects Newcastle Disease Virus genome

上海科技大学--沈庆涛

　　专家介绍：

　　沈庆涛以冷冻电子显微学作为主要手段，结合其它生物物理学手段，系统研究胞内运输中重要蛋白质的结构与功能以及病毒与宿主的互作。至今为止在国际众多著名期刊上发表高质量论文13篇，包含2篇Science，1篇Cell，1篇NSMB，1篇PNAS，2篇JCB，1篇eLife。

　　沈庆涛获得第13批中组部青年千人计划和上海市浦江人才计划资助。

　　沈庆涛目前担任中国生物物理学会监事，生物物理学学会冷冻电镜分会理事和膜生物学分会理事。

　　报告简介：

　　The nucleoprotein (N) of non-segmented negative-sense RNA viruses such as Measles, Ebola and Newcastle disease viruses (NDV) enwraps viral genomic RNA and assembles into helical filaments, serving as the template for replication and transcription. Using cryo-Electron Microscopy, we identified and resolved NDV N forming a 4.8 Å resolution clam-shaped structure with two single-turn spirals packing in a back-to-back mode, which have not been reported before. More interestingly, this clam-shaped structure can function as a seed to assemble into double-headed filaments with two separate RNAs inside. Disruption of clam-shaped structure by transition mutation on loop interface (residues 114-120) yields a single-headed filament and exposes RNA 5’ end accessible to nuclease, which will abolish viral genome replication in minigenome analysis. Our data manifest a self-coating mechanism in which clam-shaped structure and derived double-headed filaments fully protect NDV viral genome and are new potential targets for drug design.

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