导读:近日,加拿大欧罗拉生物科技有限公司在仪器信息网发布欧罗拉混合精斑DNA前处理工作站(差异裂解法)VERSA1100新品
欧罗拉差异裂解法,DNA前处理工作站,混合精斑前处理工作站
differential digestion workstation
在法医学实践中,混合斑检材以精阴混合斑最为常见,即精液与阴道分泌液的混合物。
Forensic sexual assault cases with mixed samples of semen and epithelial cells are very common.
对于此类检材,都必须采用差异裂解法进行精子细胞分离。
For such samples, differential digestion method must be used to separate sperm cells.
随着技术改进,结合脱氧核苷酸酶Dnase Ⅰ和改良的碱性裂解液,以及自动化液体工作站平台,即可轻松快速从含有精斑的混合液中获得精子DNA。
With the improvement of technology, sperm DNA can be easily and quickly obtained from the sample mixture by combining deoxynucleotidase Dnase I with improved alkaline lysate and automated liquid workstation platform.
应用:
Application:
√ 食品安全检测
_Food safety testing
√ 血液/血清中维生素D萃取
Extraction of Vitamin D from Blood/Serum
√ 公安药物实验室中滥用检测
_Detection of Toxic Substance Abuse in Public Security Drug Laboratory
√ 血液中促进生长剂的检测
_Detection of growth-promoting agents in blood
√ 水产品中禁用药物,如孔雀石绿
_Prohibited drugs in aquatic products, such as malachite green
√ 药物研发化合物纯化
_Purification of Drug R&D Compounds
√ 尿液中异黄酮分离
Detection of Isoflavonoides from urine samples
√ 海产品中的黄曲霉素检测
_Detection of Aflatoxin in seafood
√ 非挥发或半挥发分析化合物处理
_Treatment of Non-volatile or Semi-volatile Analytical Compounds
√ 食品中氯霉素
_Chloramphenicol in Food
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Applications
Genomics
· • Automated Isolation of Genomic DNA using the MACHEREY- NAGEL NucleoMag® Plant kit by Aurora Biomed’s VERSA 1100
· • Automated Isolation of Genomic DNA using the MACHEREY-NAGEL NucleoMag® Blood 200μL kit by Aurora Biomed’s VERSA 1100
· • 采用性犯罪试剂盒差异消化方法在VERSA 1100自动化应用
· • VERSA™ 1100 GENE在下一代测序(NGS)文库制备自动化的可行性验证
· • 全血样品中核酸提取应用报告
· • 植物样品中核酸提取应用报告
· • Automation of DNA Extraction
· • PCR Setup
· • Automation of Reverse Transcriptase PCR
· • Automation of Real time PCR
· • Automation of RNA Sequencing
· • Automation of Next Generation Sequencing
· • Automation of DNA Microarray
· • Automation of Miniprep
· • Automation of Sanger Sequencing
· • Automation of On-Slide (Amplislide) PCR Setup using VERSA™ 110 PCR Setup Workstation
· • Food Safety Monitoring using VERSA™ 110 NAP Workstation
· • Hot-Start PCR using VERSA™ 110 PCR Workstation
· • DNA Isolation from Saliva (Invitek Forensic DNA Isolation Kit)
· • Nucleic Acid Prep for Avian Flu Viral RNA
· • β-Actin and Whole Genome Amplification (Sigma & Promega kits)
· • Genomic DNA Isolation from Blood (Promega)
· • Automation of Molecular Pathology Applications on the VERSA™ 10 PCR Setup Workstation
· • Automated System for High Throughput PCR Setup
Extraction
· • 高通量固相萃取&气相色谱-质谱联用方法定量检测吸毒者尿液中甲基苯丙胺和苯丙胺
· • HTS Flux Assay Automation
· • Validation of Automated Liquid Liquid Extraction of 25-hydroxy vitamin D
· • Automation of Sample Preparation and Introduction into NMR Tubes
· • Liquid Liquid Extraction of β-carotene
· • Automation of Protein Purification
General Liquid Handling
· • High-Density Peptide Array Printing
· • Specimen Staining for TEM (Array printing)
· • Automated Slide-Based Assay Setup using VERSA™ 110 Workstation
· • VERSA™ Spotter Workstation for Solid-Phase Peptide Synthesis
· • Automated Protein Crystallography Plate Setup using VERSA™ 110
如何给欧罗拉留言?
欢迎点击【立即咨询】,【发送留言】后我们会马上联系您,为您的实验或应用需求推荐合适的仪器配置
创新点:
仪器针对法医学实践中的混合检材,尤其是精阴混合斑,采用特殊的差异裂解法进行精子细胞分离。 现混合精斑DNA前处理工作站,将差异裂解法在液体工作站中特色设计为自动化,结合脱氧核苷酸酶DnaseⅠ和改良的碱性裂解液,,即可轻松快速从含有精斑的混合液中获得精子DNA。 现特色模块如实际冷槽,对缓冲液、生物酶、试剂等低温保存,提高提取效率。 典型案例: 加利福尼亚州奥克兰警察局采用我司VERSA1100差异裂解法进行法医分析,并发表了论文证明了他们的成功。论文链接https://www.ncjrs.gov/pdffiles1/nij/grants/242773.pdf
来源于:仪器信息网
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