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让蛋白稳定性变得简单
如果能提前知道蛋白制剂的微小改动将如何影响蛋白分子的稳定性，或者提前知道蛋白分子会发生聚集，你会觉得怎么样？获取这些信息的唯一方式就是活的ΔG，而HUNK将ΔG的测定变得极为简单。你可以马上就知道蛋白结构、浓度以及制剂条件的变化将会对蛋白稳定性产生什么影响，而不是等到以后。
ΔG 定量检测蛋白稳定性
ΔGtrend 预测蛋白聚集路径
Kd 检测小分子亲和力
一切自动化
The Hunk completely automates the chemical denaturation and protein stability measurement process. Easy set up in application-specific trays is all it takes to get started. Once the Hunk gets going, it’ll crank out up to 96 ΔGs on 96 different conditions fully unattended. With those ΔGs in hand, you’ll know right away whether or not it’s worth spending your time on minimizing aggregation or optimizing solubility and viscosity.
一小时即得ΔG
The Hunk’s got a much cooler way to get ΔG values. It adds increasing concentrations of denaturant to proteins until they completely unfold to get their denaturation curves. The midpoint of the curve, the C1/2, is the concentration of denaturant needed to onehalf unfold the protein. The Hunk multiplies that C1/2 by the slope (m value) of the curve at the C1/2 to get the ΔG for each of your proteins — and does it in about an hour.
预测蛋白聚集
With one Hunk experiment, you’ll know if and how your protein could aggregate. Follow ΔG over increasing protein concentrations, and get the ΔGtrend. When ΔGtrend goes up, you might want to check out a native state aggregation fix. When ΔGtrend goes down, you’ve got denatured state aggregation to deal with. A flat ΔGtrend means you’re good to go. In one day, the Hunk gives you more info than ever before about your protein’s aggregation pathway.
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