串联气泡诱导喷射流动导致的细胞膜形变和生物效应

2017/06/23   下载量： 2 次

方案摘要

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应用领域 生物产业
检测样本 其他
检测项目
参考标准 暂无

利用德国LaVision公司的显微粒子成像测速分析软件包DaVis对串联气泡诱导喷射流动导致的细胞膜形变和生物效应进行了实验研究和分析。

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Cavitation with bubble–bubble interaction is a fundamental feature
in therapeutic ultrasound. However, the causal relationships
between bubble dynamics, associated flow motion, cell deformation,
and resultant bioeffects are not well elucidated. Here, we
report an experimental system for tandem bubble (TB; maximum
diameter = 50 ± 2 μm) generation, jet formation, and subsequent
interaction with single HeLa cells patterned on fibronectin-coated
islands (32 × 32 μm) in a microfluidic chip. We have demonstrated
that pinpoint membrane poration can be produced at the leading
edge of the HeLa cell in standoff distance Sd ≤ 30 μm, driven by
the transient shear stress associated with TB-induced jetting flow.
The cell membrane deformation associated with a maximum strain
rate on the order of 104 s−1 was heterogeneous. The maximum
area strain (eA,M) decreased exponentially with Sd (also influenced
by adhesion pattern), a feature that allows us to create distinctly
different treatment outcome (i.e., necrosis, repairable poration, or
nonporation) in individual cells. More importantly, our results suggest
that membrane poration and cell survival are better correlated
with area strain integral (
R
e2
Adt) instead of eA,M, which is
characteristic of the response of materials under high strain-rate
loadings. For 50% cell survival the corresponding area strain integral
was found to vary in the range of 56 ∼ 123 μs with eA,M in
the range of 57 ∼ 87%. Finally, significant variations in individual
cell’s response were observed at the same Sd, indicating the potential
for using this method to probe mechanotransduction at the
single cell level.

上一篇 在一个双稳湍流涡旋火焰中，对间歇性动态的时间-频率定位
下一篇 降低粒子成像测速中的像素锁定偏差

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北京欧兰科技发展有限公司
金牌会员 丨 第17年
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