酶、非酶分子的表面化学，光谱学研究及其应用

2019/03/06   下载量： 1 次

方案摘要

方案下载
应用领域 生物产业
检测样本 其他
检测项目
参考标准 暂无

采用立陶宛Ekspla公司的皮秒振动和频光谱测量系统（VS-SFG）对酶、非酶分子的表面化学，光谱学特性进行了实验研究并讨论了其可能的应用。

方案下载
配置单
方案详情

      The content of this thesis is an investigation into physicochemical, photophysical,
and interfacial properties of β-galactosidase, a crucial enzyme, its conjugate with CDs,
interaction of β-galactosidase with X-gal, and surface chemistry and spectroscopic study
of toxins, Cholera toxin B (CTB) and Fumonisin B1 (FB1). The objective of the work was
to understand the surface chemistry behavior of the important enzyme, conjugates and
toxins in a model systems representing vaguely cell membrane environments, to prove the
activity of the deposited enzyme, its conjugate, and toxins in a form of the monolayer. We
also studied the effect of green LED light on the FB1 Langmuir monolayer which will be
useful for possible future treatment of fungal keratitis without any side effects. Furthermore, this approach can be employed to understand the property of transmembrane receptors, to study the cell adhesion, and its implications. Besides that, this information is relevant for the successful deposition of Langmuir-Blodgett (LB) film. The Langmuir monolayer isotherm of enzyme, conjugate and the toxin were studied using the surface chemistry and spectroscopy at the air-water/subphase interphase.
      We employed the pressure (π)-area (A) isotherm measurement which is typically
the very first step in the study of these enzymatic and non-enzymatic molecules floating at
the subphase surface as it gives useful information on the existence of different phases,
phase transitions and the stability of Langmuir monolayer at fixed temperature and pH.
       During our study we found that β-galactosidase as well as its conjugate form stable
Langmuir monolayer. We observed that during the interaction of the enzyme with X-gal,
its secondary structure changes. This does not mean that the overall structure of the enzyme
gets changed. This is because enzyme structure is determined by primary, secondary,
tertiary, and quaternary structure. The interfacial study of CTB showed that the molecule
is very stable at the air-subphase interface. In performing surface chemistry and
spectroscopic study of FB1 molecule, the interesting finding that came out of the FB1
Langmuir monolayer investigation was that the analyte adopted stability (without
irradiation of green LED light) at the monolayer and remained degraded with subsequent
effect of the green LED light (525 nm) at air-water interface.

上一篇 在一个双稳湍流涡旋火焰中，对间歇性动态的时间-频率定位
下一篇 小水合物镁团簇Mg+(H2O)n, n = 1–5的光化学和光谱研究

文献贡献者

北京欧兰科技发展有限公司
金牌会员 丨 第17年
查看全部资料
推荐方案
更多

相关产品

当前位置： 仪器信息网 欧兰科技 方案 酶、非酶分子的表面化学，光谱学研究及其应用

关注

拨打电话

留言咨询