时间分辨X射线散射法探测全长蓝藻植物色素的光诱导结构改变

2019/03/09   下载量： 3 次

方案摘要

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应用领域 生物产业
检测样本 其他
检测项目
参考标准 暂无

采用立陶宛Ekspla公司的5ns脉宽可调谐激光器的630nm，0.5mJ输出光解蓝藻植物色素分子，并采用时间分辨X射线散射法探测光诱导结构改变。

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方案详情

Phytochromes are photoreceptor proteins that transmit a light signal from a photosensory
region to an output domain. Photoconversion involves protein conformational changes whose
nature is not fully understood. Here, we use time-resolved X-ray scattering and optical
spectroscopy to study the kinetics of structural changes in a full-length cyanobacterial
phytochrome and in a truncated form with no output domain. X-ray and spectroscopic signals
on the μs/ms timescale are largely independent of the presence of the output domain. On
longer time-scales, large differences between the full-length and truncated proteins indicate
the timeframe during which the structural transition is transmitted from the photosensory
region to the output domain and represent a large quaternary motion. The suggested independence of the photosensory-region dynamics on the μs/ms timescale defines a time
window in which the photoreaction can be characterized (e.g. for optogenetic design)
independently of the nature of the engineered output domain.

上一篇 在一个双稳湍流涡旋火焰中，对间歇性动态的时间-频率定位
下一篇 激光探针诊断线阵列Z-箍缩过程

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北京欧兰科技发展有限公司
金牌会员 丨 第17年
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