方案摘要
方案下载应用领域 | 医疗/卫生 |
检测样本 | 其他 |
检测项目 | |
参考标准 | 暂无 |
采用立陶宛Ekspla公司纳秒高重复频率波长可调谐纳秒Nd:YAG激光器泵浦的光学参量发生器,型号NT242-1k-SH/SFG; 用来进行时间分辨单态氧检测,同时对碘化钾增强玫瑰红介导的抗菌光动力失活过程进行了活体外和活体内研究。
Rose bengal (RB) is a halogenated xanthene dye that has been used to mediate antimicrobial photodynamic inactivation for several years. While RB is highly active against Gram-positive bacteria, it is largely inactive in killing Gram-negative bacteria. We have discovered that addition of the nontoxic salt potassium iodide (100 mM) potentiates green light (540-nm)-mediated killing by up to 6 extra logs with the Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, the Gram-positive bacterium methicillin-resistant Staphylococcus aureus, and the fungal yeast Candida albicans. The mechanism is proposed to be singlet oxygen addition to iodide anion to form peroxyiodide, which decomposes into radicals and, finally, forms hydrogen peroxide and molecular iodine. The effects of these different bactericidal species can be teased apart by comparing the levels of killing achieved in three different scenarios: (i) cells, RB, and KI are mixed together and then illuminated with green light; (ii) cells and RB are centrifuged, and then KI is added and
the mixture is illuminated with green light; and (iii) RB and KI are illuminated with
green light, and then cells are added after illumination with the light. We also showed that KI could potentiate RB photodynamic therapy in a mouse model of skin abrasions infected with bioluminescent P. aeruginosa.
在一个双稳湍流涡旋火焰中,对间歇性动态的时间-频率定位
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