信号素3A(SEMA3A)重组蛋白

信号素3A(SEMA3A)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位分泌

预测分子量13.8kDa

实际分子量14kDa(差异分析请参阅说明书)

片段与标签Pro580~Thr664 with N-terminal His Tag

缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）

性状冻干粉

纯度> 90%

等电点7.2

信号素3A(SEMA3A)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling

RPL882Hu01 100μg

Recombinant UL16 Binding Brotein 2 (ULBP2)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Gly2~Ile246

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9BZM5

Host: E. coli

Subcellular Location: Cell membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 28.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

信号素3A(SEMA3A)重组蛋白[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

RPL907Ra01 100μg

Recombinant N-myc Downstream Regulated Gene 2 (NDRG2)

Organism Species: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Ala2~Pro300

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q8VBU2

Host: E. coli

Subcellular Location: Cell projection. Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 4.8

Predicted Molecular Mass: 36.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

信号素3A(SEMA3A)重组蛋白[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

AELQEVQIT EEKPLLPGQT PEAAKEAELA ARILLDQGQT HSVETPYGSV TFTVYGTPKP

KRPAIFTYHD VGLNYKSCFQ PLFQFGDMQE IIQNFVRVHV DAPGMEEGAP VFPLGYQYPS

QDQLADMIPC ILQYLNFSTI IGVGVGAGAY ILSRYALNHP DTVEGLVLIN IDPNAKGWMD

WAAHKLTGLT SSIPEMILGH LFSQEELSGN SELIQKYRSL ITHAPNLENI ELYWNSYNNR

RD L N RPL917Hu01 10μg

Recombinant Semaphorin 3A (SEMA3A)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Pro580~Thr664

Tags: N-terminal His-Tag

Tissue Specificity: Brain, Lung, Pancreas. Subcellular Location: Secreted. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.2

Predicted Molecular Mass: 13.8kDa

Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. Gene Sequencing (Extract)

Figure 2. SDS-PAGER G GE MT LK C P VM LV VG D QA P HE DA VV E C NS K L D P TQ TS F L KMA D

SGGQPQLTQP