供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL930Hu01 |
CAS号: |
信号素3F(SEMA3F)重组蛋白来源原核表达
宿主E.coli
内毒素水平<1.0EU per 1μg (determined by the LAL method).
亚细胞定位分泌
预测分子量16.6kDa
实际分子量16kDa(差异分析请参阅说明书)
片段与标签Val350~Leu483 with N-terminal His Tag
缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状冻干粉
纯度> 95%
等电点9.2
信号素3F(SEMA3F)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
Recombinant Semaphorin 3F (SEMA3F)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Val350~Leu483
Tags: N-terminal His-Tag
Homology: Mouse 96%, rat 96%
Tissue Specificity: Breast, kidney, brain, lung, liver. Subcellular Location: Secreted. Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.2
Predicted Molecular Mass: 16.6kDa
Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
信号素3F(SEMA3F)重组蛋白[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
信号素3F(SEMA3F)重组蛋白[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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