| 供货周期： | 现货 |
| 品牌： | 康朗生物 |
| 规格： | 100ul/50ul/25ul |
| 货号： | KL310064 |
| CAS号： |
Anti-p44抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
310064Anti-p44/42 MAP Kinase(Phospho-Thr202) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC42 44
Sensitivity The antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at threonine 202.
Anti-p44抗体Immunogen Peptide sequence around phosphorylation site of threonine 202 (F-L-T(p)-E-Y) derived from Human p44/42 MAP Kinase.
Host Rabbit
Clone Number
Anti-p44抗体Antibody type Polyclonal antibody
Purified method
Isotype IgG
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution
Anti-p44抗体WB~~1:500-1:1000
ICC~~1:100-1:200
IHC~~1:50-1:100
Application Images-expand |
Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using p44/42 MAP Kinase (Phospho-Thr202) Rabbit pAb (310064,1:500 diluted,upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246,1:1000 diluted,lower).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase (Phospho-Thr202) Antibody .
Western blot analysis of extracts from 293 cells untreated or treated with PMA for the indicated times, using p44/42 MAP Kinase (Phospho-Thr202) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Thr202) Antibody (left) or the same antibody preincubated with blocking peptide #51245 (right).
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