供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 100ul/50ul/25ul |
货号: | KL600472 |
CAS号: |
Anti-Histone H2A.X抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
600472Anti-Histone H2A.X Mouse mAbMouse mAbHuman,Rat.WB, IHC-P,IF15kDa
Sensitivity
Anti-Histone H2A.X抗体Immunogen This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX.
Host Mouse
Clone Number 4C5-E2-D8
Antibody type Monoclonal antibody
Anti-Histone H2A.X抗体Purified method Affinity purified
Isotype IgG1
Formulation Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Anti-Histone H2A.X抗体Dilution
WB~~1:1000
IHC~~1:25
Application Images-expand |
Western blot detection of Histone H2A.X in MCF7,C6 and Hela cell lysates using Histone H2A.X mouse mAb (1:10000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Histone H2A.X mouse mAb (dilution 1:200).
Immunohistochemical analysis of paraffin-embedded H. prostate section using H2AFX Antibody (N-term)(Cat#600472). 600472 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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