供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 50μl |
货号: | ZY-1911R |
CAS号: |
Anti-IL12 antibody
种属反应性Mouse,Rat
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from mouse IL-12 51-150/215
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Secreted.
数据链接SwissProt: P43431 Mouse
其它名称
WB:1:500-2000
IHC-P:1:400-800
FC:1μg/Test
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (ER1911-21) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 (ER1911-21) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
Fig3: Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody (ER1911-21)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (ER1911-21) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
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