供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 50μl |
货号: | ZY-1911R |
CAS号: |
Anti-IL-5 antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,ICC/IF
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from mouse IL-5 56-132/132
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Secreted.
数据链接SwissProt: P04401 Mouse
其它名称
WB:1:500-2000
IHC-P:1:400-800
IF:1:100-500
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (ER1911-38) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (ER1911-38) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig3: Sample:
Lovo Cell (Human) Lysate at 30 ug
Primary: Anti- IL-5 (ER1911-38)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13kD
Observed band size: 10kD
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
相关产品
彩色预染蛋白Marker(10-180 kDa,三色)
Super GelBlueTM 核酸染料, 10,000× in water
彩色预染蛋白Marker(10-180 kDa,三色)
彩色预染蛋白Marker(10-250kDa, 双色)
琼脂糖 A2015
Super ECL Plus(超敏化学发光检测试剂盒)
PAGE 彩色快速凝胶制备试剂盒(10%)
Minerva Super Fusion Cloning Kit(无缝克隆试剂盒)
Cell Counting Kit-8(CCK-8)细胞增殖检测试剂盒
FITC-Annexin V/PI 细胞凋亡试剂盒
呋喃西林快速检测卡
呋喃妥因快速检测卡
呋喃它酮快速检测试剂盒
呋喃妥因快速检测试剂盒
呋喃西林快速检测试剂盒
关注
拨打电话
留言咨询