供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY-6803-83R |
CAS号: |
Anti-Estrogen Receptor alpha antibody
种属反应性Human, Mouse, Rat
验证应用WB,FC
抗体类型兔多抗
免疫原Recombinant protein within Human Estrogen Receptor alpha aa 1-120.
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Cytoplasm. Nucleus. Cell membrane. Golgi apparatus.
其它名称
WB: 1:500-1:1,000
FC: 1:50-1:100
Fig1: Western blot analysis of Estrogen Receptor alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293T cell lysate
Lane 2: Rat ovary tissue lysate
Fig2: Flow cytometric analysis of Estrogen Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with Estrogen Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
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