供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY-6907-87R |
CAS号: |
Anti-E2F5 antibody
种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human E2F5 101-200/346
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus.
其它名称
WB:1:500-2000
IHC-P:1:400-800
FC:1μg /test
Fig1: Sample:
RSC96(Rat) Cell Lysate at 30 ug
Primary: Anti-E2F5at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 34 kD
Fig2: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
Fig3: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (E2F5) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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