供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY6906-94R |
CAS号: |
Anti-Cyclin E1 antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,ICC/IF,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from rat Cyclin E 375-411/411
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus.
其它名称
WB:1:500-2000
IHC-P:1:400-800
FC:1μg/Test
IF:1:100-500
Fig1: Sample:
Brain(Rat) lysate at30ug;
Lung(Rat) lysate at 30ug;
Primary: Anti-Cyclin E at 1:200;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;
Predicted band size : 45kD
Observed band size : 45kD
Fig2: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin-E Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Cell: NIH/3T3
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of primary antibody on NIH/3T3(green) compared with Rabbit IgG isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
Fig4: Sample: Hela Lysate at 40 ug
Primary: Anti-Cyclin E at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
Fig5: Sample:
Brain(Mouse) Lysate at 40 ug
Testis(Mouse) Lysate at 40 ug
Primary: Anti-Cyclin Eat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
Fig6: Blank control (blue line): Mouse spleen cells (blue).
Primary Antibody (green line): Rabbit Anti-Cyclin E1 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig7: Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-Cyclin E1at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
Fig8: Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin E1 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
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