供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY-6912-84R |
CAS号: |
Anti-MC4 Receptor antibody
种属反应性Human,Mouse,Rat
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human MC4 Receptor 1-50/332
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式Affinity purified by Protein A
亚细胞定位Cell membrane; Multi-pass membrane protein.
其它名称
WB:1:500-2000
FC:1μg/Test
Fig1: Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-MC4 Recepto at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 40 kD
Fig2: Positive control: RSC96
Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μg in 100 μL1X PBS containing 0.5% BSA.
Fig3: Blank control: 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-MC4 Receptor /AF488 Conjugated antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Fig4: Blank control: 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-MC4 Receptor /AF488 Conjugated antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Fig5: Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- MC4 Receptor at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 52 kD
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