供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY600005R |
CAS号: |
Anti-p21 ARC antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC
抗体类型兔多抗
免疫原Synthetic peptide within human p21 ARC aa 120-178.
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Cell projection, Cytoplasm, Cytoskeleton, Nucleus.
其它名称
Actin related protein 2/3 complex subunit 3 antibody
ARC21 antibody
ARP2/3 complex 21 kDa subunit antibody
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:100
Fig1: Western blot analysis of p21 ARC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: Rat lung tissue lysate
Lane 3: Mouse lung tissue lysate
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-p21 ARC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p21 ARC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodyfor 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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