供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 小鼠单抗 50 μl |
货号: | ZY60068M |
CAS号: |
Anti-eIF4A1 antibody
种属反应性Human, Mouse, Rat
验证应用WB, IHC-P, IF
抗体类型小鼠单抗
免疫原Synthetic Peptide of eIF4A1
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
亚型IgG1
纯化方式Immunogen affinity purified.
亚细胞定位Cytosol, Extracellular region or secreted.
其它名称
ATP-dependent RNA helicase eIF4A-1 antibody
DDX2 antibody
DDX2A antibody
WB: 1:1000-3000
IF: 1:100-200
IHC-P: 1:50-300
Fig1: Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1,eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Fig2: Immunofluorescence analysis of Mouse-spleen tissue. 1,eIF4A1 Monoclonal Antibody(M8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Fig3: Western blot analysis of 1) 293T, 2) Hela, 3) HepG2, 4) Mouse Brain tissue,
Fig4: IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
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