供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 小鼠单抗 50 μl |
货号: | ZY6710-57M |
CAS号: |
Anti-P2RY12 antibody
种属反应性Human
验证应用WB,FC
抗体类型小鼠单抗
免疫原Purified recombinant fragment of human P2RY12 (AA: extra mix) expressed in E. Coli.
偶联Non-conjugated
形态Liquid
浓度1 mg/mL
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS with 0.05% sodium azide.
亚型IgG1
纯化方式Protein G purified.
亚细胞定位Cell membrane.
其它名称
ADP glucose receptor antibody
ADP-glucose receptor antibody
ADPG R antibody
WB: 1:500-1:2,000
FC: 1:100-1:200
Fig1: Western blot analysis of P2RY12 against human P2RY12 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of P2RY12 against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig3: Western blot analysis of P2RY12 against PC-3 (1) and C6 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig4: Flow cytometric analysis of P2RY12 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody(green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
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