供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 小鼠单抗 50 μl |
货号: | ZY6711-10M |
CAS号: |
Anti-TNFRSF6B antibody
种属反应性Human
验证应用WB,FC
抗体类型小鼠单抗
免疫原Purified recombinant fragment of human TNFRSF6B (AA: 30-300) expressed in E. Coli.
偶联Non-conjugated
形态Liquid
浓度1 mg/mL
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS with 0.05% sodium azide.
亚型IgG1
纯化方式Protein G purified.
亚细胞定位Secreted.
其它名称
DcR3 antibody
Decoy receptor 3 antibody
Decoy receptor for Fas ligand antibody
WB: 1:500-1:2,000
FC: 1:100-1:200
Fig1: Western blot analysis of TNFRSF6B against human TNFRSF6B (AA: 30-300) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of E against HEK293 (1) and TNFRSF6B (AA: 30-300)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig3: Flow cytometric analysis of TNFRSF6B was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody ((green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
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