供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 小鼠单抗 50 μl |
货号: | ZY6711-93M |
CAS号: |
Anti-NFKB2 antibody
种属反应性Human,Mouse
验证应用WB,FC
抗体类型小鼠单抗
免疫原Purified recombinant fragment of human NFKB2 (AA: 712-900) expressed in E. Coli.
偶联Non-conjugated
形态Liquid
浓度1 mg/mL
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS with 0.05% sodium azide.
亚型IgG1
纯化方式Protein G purified.
亚细胞定位Cytoplasmic and Nuclear.
其它名称
CVID10 antibody
DNA binding factor KBF2 antibody
H2TF1 antibody
WB: 1:500-1:2,000
FC: 1:100-1:200
Fig1: Western blot analysis of NFKB2 against human NFKB2 (AA: 712-900) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of NFKB2 against HEK293 (1) and NFKB2 (AA: 712-900)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig3: Western blot analysis of NFKB2 against NIH/3T3 (1), MCF-7 (2), and THP-1 (3) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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