供货周期: | -- |
品牌: | 百欧博伟生物 |
规格: | 冻干粉 |
货号: | bio-134268 |
CAS号: | ATCC VR-1928 |
平台编号:Bio-134268
Rickettsia monacensis拉丁名 VR-1928™统一编号
Type strain. Genome sequenced by depositor.
Product category Bacteria
Classification Rickettsiaceae, Rickettsia
Strain designation IrR/Munich
Type strain Yes
Genome sequenced strain Yes
Isolation source Tick (Ixodes ricinus), isolated on June 12, 1998.
Geographical isolation Germany; Munich
Applications Infectious disease research
Vector-borne disease research
Product format Frozen
Storage conditions Vapor phase of liquid nitrogen
Comments
Genome sequenced by depositor
Handling information
Host
Vero (ATCC CCL-81)
Effects
CPE; cell rounding; cell sloughing; cell degeneration
Complete medium
DMEM (ATCC® 30-2002™) + 5% FBS (ATCC® 30-2020™)
Temperature
34°C
Incubation
6-11 days at 34°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. A Giemsa stain may also be used to determine end of incubation.
Recommendation for infection
Plate cells 18-24 hours prior to infection and infect when cultures are 65-75% confluent. Remove DMSO from bacteria vial by centrifugation (180 x g for 10 minutes at 4°C). Remove supernatant and add 2 mL of fresh medium to the pellet. Pulse vortex for 1 minute. Remove medium from host cell culture and inoculate with bacteria (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 1). Adsorb by centrifugation for 30 minutes at 800 x g at 22°C. Add agent growth medium following adsorption.
Key abbreviations
°C, Degrees Celsius
DMSO, Dimethyl sulfoxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
Volume
1.0 mL
History
Deposited as
Rickettsia monacensis
Depositors
T Kurtti, University of Minnesota
Chain of custody
ATCC <-- T Kurtti
Type of isolate
Arthropod
Year of origin
1998
Cross references
GenBank NZ_LN794217.1 Rickettsia monacensis, strain IrR/Munic, complete genome.
相关产品
单孢共头霉原变种
Thamnostylum Repens
Zychaea Mexicana
Piptocephalis Cylindrospora
Piptocephalis Indica
Piptocephalis Repens
Companilactobacillus nodensis
Companilactobacillus nantensis
Companilactobacillus mishanensis
Companilactobacillus mindensis
Companilactobacillus metriopterae
Companilactobacillus kimchiensis
Companilactobacillus kedongensis
Companilactobacillus jidongensis
Companilactobacillus hulinensis
关注
拨打电话
留言咨询