产品详情
GSK3B Protein, Mouse, Recombinant (His Tag): 产品信息
纯度
> 75 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. The specific activity was determined to be > 20 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.
2. Immobilized His-mGSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1 (cat:11279-H20B), EC50 of biotinylated human HG3C-CTNNB1 (cat:11279-H20B) is 0.15-0.35 μg/ml.
蛋白构建
A DNA sequence encoding the mouse GSK3B (Q9WV60) (Met 1-Thr 420) was fused with a polyhistidine tag at the N-terminus.
表达宿主
Baculovirus-Insect Cells
分子量
The recombinant mouse GSK3B consists of 439 amino acids and has a calculated molecular mass of 49 kDa. It migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
缓冲液
Supplied as sterile 20mM Tris, 500mM NaCl, 25% glycerol, 0.2mM DTT, pH 7.4
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研，不能用于临床诊断！所有产品仅供科研使用，不得用于人或动物的治疗等任何其他用途，不为任何个人提供产品和服务。
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