产品详情
GFR Alpha-1/GFRA1 Protein, Human, Recombinant (His Tag): 产品信息
纯度
> 98 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured in a cell proliferation assay using SH?SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.
2. Measured by its ability to bind human GDNF (native) in functional ELISA.
蛋白构建
A DNA sequence encoding the human GFRα1 without the propeptide (NP_665736.1) (Met 1-Ser 424) was fused with the a polyhistidine tag at the C-terminus.
分子量
The recombinant human GFRα1 consists of 411 amino acids after removal of the signal peptide and predicts a molecular mass of 46 kDa. As a result of glycosylation, the rhGFRα1 migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研，不能用于临床诊断！所有产品仅供科研使用，不得用于人或动物的治疗等任何其他用途，不为任何个人提供产品和服务。
GFR Alpha-1/GFRA1 Protein, Human, Recombinant (His Tag)信息由上海博尔森生物科技有限公司为您提供，如您想了解更多关于GFR Alpha-1/GFRA1 Protein, Human, Recombinant (His Tag)报价、型号、参数等信息， 欢迎来电或留言咨询。除供应GFR Alpha-1/GFRA1 Protein, Human, Recombinant (His Tag)外，上海博尔森生物科技有限公司还可为您提供CD47 Protein, Mouse, Recombinant (His Tag)、PDGFRA Protein, Mouse, Recombinant (ECD, His Tag)等产品， 公司有专业的客户服务团队，是您值得信赖的合作伙伴。
