产品详情
RANKL Protein, Human, Recombinant (mFc Tag): 产品信息
纯度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF11B His (Cat:10271-H08H) at 2 μg/ml (100 μl/well) can bind Human RANKL mFc(Cat:11682-H04H), the EC50 of Human RANKL mFc is 15-80 ng/mL.
2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 15-45 ng/mL.
蛋白构建
A DNA sequence encoding the human TNFSF11 (AAC51762.1) (Gly63-Asp244) was expressed with the Fc region of mouse IgG1 at the N-terminus.
分子量
The recombinant human TNFSF11/mFc comprises 418 amino acids and has a predicted molecular mass of 47.1 kDa. The apparent molecular mass of the monomer is approximately 50-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研,不能用于临床诊断!所有产品仅供科研使用,不得用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务。
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