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Four flavonoids, isoastilbin, astilbin, isoengelitin, and engelitin were isolated and purified simultaneously from Smilacis Glabrae Roxb. for the first time by high performance counter-current chromatography using a system consisting of n-hexane–n-butanol–water (1:2:3, v/v/v). A total of 392.6 mg of astilbin, 71.4 mg of isoastilbin, 47.4 mg of engelitin, and 10.3 mg of isoengelitin were purified from 1.89 g of the ethyl acetate extract of Smilacis Glabrae Roxb. in six runs, each at over 94.51% purity as determined by HPLC. The structures of the four compounds were identified by their retention time, the LC-ESI-MSn in the negative ion mode, and confirmed by 1H-NMR experiments. The characteristic LC-ESI-MS fragmentation patterns of the four compounds were discussed.
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弗士达仪器为您提供《应用高效逆流色谱同时分离两类顺反异构体》，该方案主要用于其他中null检测，参考标准《暂无》，《应用高效逆流色谱同时分离两类顺反异构体》用到的仪器有高效/高速逆流色谱HPCCC/HSCCC/CCC/CPC中药现代化。
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