樟脑醌类化合物中CPL光谱检测方案(圆二色光谱仪)

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Application NoteCD-0031 CPL Measurement of Camphorquinone Using CPL-3002/3Application NoteCircularly Polarized Luminescence Spectrometer CPL Measurement of Camphorquinone Using CPL-300Circularly Polarized Luminescence Spectrometer When chiral compounds are excited with unpolarized light，the difference in emission intensities of left- and right-handedcircularly polarized light can be measured. This phenomenonis called circularly polarized luminescence (CPL). While circulardichroism provides information about the ground state of chiralmolecules， CPL spectroscopy probes the excited states ofchiral molecules. CPL-300 Circularly Polarized Luminescence Spectrophotometer By combining CPL with ECD， more structural information regarding chiral molecules can be obtained. To measure sharpCPL peaks at a high resolution， the CPL spectrometer uses two prism monochromators. Both the emission and excitationmonochromators are equipped with continuously variable slit drives， which allow for an appropriate wavelength and bandwidth selection. This application note demonstrates the measurement and analysis of camphorquinone using two complimentarytechniques： circular dichroism and circularly polarized luminescence. Keywords Circularly polarized luminescence， Circular dichroism， CD-0031， Materials Experimental 18 mM of (1R)-(-) - and (1S)-(+)-camphorquinone was prepared in ethanol. Measurement Conditions CD CPL Data Pitch 0.1nm Excitation Wavelength 440nm Scan Speed 50 nm/min Excitation Bandwidth 16 nm D.I.T. 2 sec Data Pitch 0.1 nm Bandwidth 1nm Emission Bandwidth 10mm Accumulations 1 Scan Speed 50 nm/min D.I.T. 4 sec Accumulations 16 Results The CPL and fluorescence spectra of (1R)-(-)- and (1S)-(+)- camphorquinone were measured using a CPL-300， while CDand absorption were collected using a J-1500. The CD and CPL signals were normalized as gabs=Ae/e and g um=AI/I，respectively. The spectra are shown in Figure 1. (a) 0.0125 0.003 (C) 0.002 0.0075 0.001 0.0025 0 -0.0025 --0.001 -0.0075 -0.002 -0.0125 -0.003 250 300 350 400 450 500 550 600 Wavelength (nm) Wavelength (nm) Figure 1. The (a) CD and CPL spectra， (b) absorbance and fluorescence spectra， and (c)g and gspectra of the(1R)-(-)-(bluered) and (1S)-(+)-(bluered) camphorquinone ethanol solution. 28600 Mary's Court， Easton， MD 21601 USA Application Note Conclusion The results are in good agreement with the camphorquinone CPL spectrum previously reported. 1. Chun Ka Luk and F. S. Richardson，JACS.1974，96，2006-2009. 28600 Mary's Court， Easton， MD 21601 USA JASCO INC.Mary's Court， Easton， MD USATel： ( Fax： ( pplication Library： http：//www.jascoinc.com/applications This application note demonstrates the measurement and analysis of camphorquinone using two complimentary techniques: circular dichroism and circularly polarized luminescence.