糖醇的HPLC-ELSD分析

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蒸发光散射检测器（ELSD）是一种通用的HPLC检测器，其检测原理基于光散射现象。在使用热去除挥发性流动相并用氮气喷射之后，当光照射到残留的非挥发性组分的颗粒上时发生散射。在检测部分中，LED被用作照射非易失性组件的光源，并且散射光被光电倍增器转换为电信号以测量强度。糖和脂肪通常使用折射率检测器或紫外线检测器的短波长范围进行测量，可以以更高的灵敏度和更稳定的基线进行测量。2/3 3/3Analysis of Sugar Alcohols by HPLC-ELSDApplication Note Application Not e JS.cO Analysis of Sugar Alcohols by HPLC-ELSD An evaporative light scattering detector (ELSD) is a universal HPLC detector whose detection principle is based the light scattering phenomenon. The scattering occurs when l ight is i rradiated on to particles of residual non-volatile components after removing the volatile mobile phase using heat and sprayed wi t h nitrogen gas. In detection portion， LED is used as the light source for irradiating the non-volatile components and scattered light i s converted into an electrical signal by the photomult i plier to measure the intensity. Sugar and fat， which had been usually measured using a refractive index detector or a short wavelength range of a UV detector， can be measured with higher sensitivity and a more stable baseline. In this report sugar alcohols were measured using ELSD as a detector and a polymer NH2 column in HILIC mode PU-2089 Keywords： ： sugar alcohols， HILIC， polymer NH，column， ELSD， Application Note A n a lysis of S ugar A l c o h ol s by H P L C -EL S D E xperimenta l Equipment Condit i ons Pump： PU-2089 Autosampler： AS-2057 Column oven： CO-2060 Detector： ELS-2040 Column： Shodex Asahipak NH2P-50 4E (4.6 mmlDx250 mmL) Eluent A： Water/Acetonitrile (25/75) Flow rate： 1.0 mL/min Column temp.： 30°℃ FELSD condition： Nebulizer temp.： 30℃Evaporator temp.： 30℃ Gas flow rate； 1.4 SLM Injection volume： 10pL Standard sample： Xylitol， Mannitol， Inositol， Maltitol 1.0 mg/mL each in Water/ Acetonitrile (50/50) Copyrig h t O JASCO Co r por ati on The chromatogram of a standard mixture of sugar alcohols i s shown in f igure 1. Figure 1. Chromatogram of standard mixture of sugar alcohols. 1： Xylitol， 2： Mannitol， 3： Inositol， 4： Maltitol