检测样品 生物药品药物研发
检测项目 分子量、SEC、GPC计算程序、
方案详情文
智能文字提取功能测试中
硫酸软骨素(分子量20000~50000)(其中一种是粘多糖)存在于动物结缔组织软骨和皮肤中,有几种类型(A至E)。硫酸软骨素广泛应用于保健食品和药品的生产。在本申请中,硫酸软骨素(钠盐)使用排除极限为300000分子量的SEC(Size Exclusion Chromatography)柱(Pullulan)进行分析,并使用折射率(RI检测器)进行检测。使用ChromNAV和可选的GPC计算程序从标准混合物中创建Pullulan校准曲线,并且由普鲁兰标准计算得到的所得分子量如本应用所述。Application Note 2/4 Analysis of Chondroitin Sulfate Sodium Salt by SEC C Introduction There are several types (A to E) of chondroi ti n sulfate (MW 20,000~50,000) (one of which is a mucopolysaccharide)which are found in ani m al connective tissue -cartilage and ski n . Chondroitin sulfate is widely used in t he manufacture of health foods and medicines. In this application note Chondroitin sulfate (sodium salt ) was ana l yzed using a SEC (Size Exc l usion Chromatography) column with an excl u sion limit of 300,000molecular weight (Pullulan) and detection using refract i ve index (RI detector). A Pul l ulan calibration curve was created from a standard mixture using ChromNAV with the optional GPC Calcul a tion Program and t he resulting molecular weights calculated from the Pullulan stand a rd are described below. JASCO Re fr act iv e Index Detector at www.jascoi n c.co m 28600 Mary's Court, Easton, MD 21601 USA Analysis of Chondroitin Sulfate Sodium Salt by SEC Application Note Exper i mental Equipment Conditions Pump: PU-2080 Degasser: DG-2080-53 Column Oven: CO-2060 Autosampler: AS-2057 Detector: RI-2031 Column: Shodex Asahipak GF-510HQ (7.5 mml.D.x300 mmL, 5) um Flowrate: Column Temp.: Structure n PL-STD1 PL-STD2 Grade Mp Grade Mp P-100 P-20 P-5 107,000 21,100 5,900 P-200P-50 P-10 200,000 47,100 9,600 Chondroi ti n Sulfate C Sample: 0.1% Chondroit i n sul f ate C sodium salt in eluent Results Fig. 1 shows the chromatograms of Pullulan (PL), standard mixture for molecular weight calibrat i on and chondroi ti n sulfa t e sod iu m s a lt. Fi g. 1. Chromatograms of Pullulan (PL ), Standard Mixtur e for Molecular Weight Calibration and Chondroi t in Sulfat e Sodium Sali (Each value o n t h e chromat ogr ams is Mp (Peak top m olecular weig ht).) Fig. 2 shows molecular weight calibration curve created with Pullulan as a standard mixture. Fig. 3 shows a chromatogram of chondroitin sulfate sodium salt and molecular weight calibration curve. Fig. 4 and Table 1 shows molecular weight distribution calculation curve and the result of molecular weig h t calculated with Pullulan respectively. Fig . 2. Molecular Weight Calibrat i on Curve Created with PL Standard Mixture Fig. 3. Chromatogram of Ch o ndroit i n Sulfate Sodium Salt a n d Molecular Weight Cali b rat i on Curve (T he f igure i n blue represents Mp calculated with Pullulan .) Fig . 4. Mol e cular We i ght Distribution Curve of Chondroitin Sulfate Sodium Salt T able 1. Pullulan Conve r ted Molecular Wei gh t of Chondroit i n Sulfate Sodium Salt Mp Mn Mw Mz Mv Mw/Mn Mz/Mw 46812 36668 57914 85947 57914 1.58 1.48
还剩2页未读,是否继续阅读?
继续免费阅读全文产品配置单
佳士科商贸有限公司为您提供《硫酸软骨素的Size Exclusion Chromatography》,该方案主要用于生物药品药物研发中分子量、SEC、GPC计算程序、检测,参考标准《暂无》,《硫酸软骨素的Size Exclusion Chromatography》用到的仪器有JASCO高效色谱仪LC-4000。
我要纠错相关方案