高效液相色谱-紫外-荧光检测法检测柴油机尾气中的硝基芳烃

智能文字提取功能测试中

汽车尾气中存在一类被称为硝基芳烃的多环芳烃。已知这些物种具有高度致突变性和致癌性。因此，开发良好的方法来分析这些物种是非常重要的。在许多情况下，干扰化合物的存在往往掩盖了目标化合物，使得高选择性和灵敏的检测变得困难。在本实验中，采用了在线还原系统和净化方法来增强荧光并提供更高的选择性。该系统允许同时分析柴油废气中的多环芳烃和硝基芳烃。Experimental：在配备有NPpak-P预处理柱、用于分离的NPpak柱和用于还原的NPpak-R柱的Jasco HPLC上运行一系列掺有硝基芳烃的柴油废气样品。样品采用甲醇和水以1mL/min的流速梯度洗脱。预处理和分离温度为40°C，还原温度为100°C。使用10µL的进样体积和254nm的紫外检测，对含有五种标准品（萘、苊、蒽、氟和蒽）的样品和四种柴油废气样品进行了检测。使用荧光检测器以365nm的激发在436nm处监测发射。Detection of Nitroarenes in Diesel Exhaust Using HPLC with UV2/2Application Noteand Fluorescence Detection Application Not e JAS.C O Detection of Nitroarenes in Diesel Exhaust Using HPLC with UV and Fluorescence Detection In t roduct i on A f am i l y of po l ya r o m a ti c h ydrocarbo n s (P A H s) known as nitroaren e s are p re se n t in automobile exha u st. T hes e spec i es a r e know n to be high ly mutagenic a n d ca r cinogen i c . T h us，i t is ve r y i mpo r tant to deve l op good methods f or the a n alys i s of t h ese species. I n ma ny cases t h e p rese n ce of i nterfering compound s o ft en ma sk s t h e t arge t compo und s， ma k ing high l y sele c tive and sensi ti ve d etec ti on diffi cult . I n t hi s ex p e r i m ent，a n o n -l i n e reduc t io n sy st em and c l e an-u p m e tho d i s em plo y e d to enh a nce t he fl uo r e s c ence and pr ov i d e hi g he r s el e c t i vity.T he s yst em a ll ows simult an eo us ana ly s is of PAH s and nitroarenes in d i esel ex h aus t . Experime n ta l A series o f d i esel ex ha us t samples s pik ed wi th nitr oa ren es we r e r un on a Jasco H PLC e quippe d with a n NPp ak -P pretreatment co lu mn， an NPp ak co lu mn f or sepa r ation， and an N P p ak -R column fo r r edu cti on. Samples were ru n us i n g g r a d ien t e luti on with methano l a nd water a t a f l ow rate of 1m L /m i n. P r etrea t m e nt and sepa r a t i o n tempe r ature was 40℃whil e reductio n wa s do ne a t 100℃. A sa m p l e c on ta ini n g f i v e s t andar d s， n aphtha l ene， a cena pthy l ene ， ace na pthen e ，fluo r ine， a n d p ena nth r en e a n d fo u r di e s e l e xha us t samp le s w e r e r un u s ing an i nje ctio n vol u m e o f 10pL and U V de t ect i on at 254nm. A fluo r escence detec t or was used mo n i t ori n a em i ssio n a t 436nm with a n excita t io n of 365n m . J a sc o LC 2000 for detect i on of Ni t r o are n e s Fi g ure 1 sh o ws the c h r omat o g r am o f t h e 5 component n it r oa r e n e standard. Using t h e HPLC system d e scr i bed above w i th f luo r esce n ce detect i o n， a ll 5 components we r e a ble to be separated and d ete c t ed.Fi g ur e 2 shows f l u o re s ce n c e d etec t io n of n i t r o pyre ne (NP) in di e sel e xha u st . Altho u gh a l a rge number o f spe c ies a r e de t e c t e d， n o ne o f them i n te r fe r e s w i t h the n i tropyr e ne p e ak w h i ch e l u t e s i n a pproxima t e ly 23mi n utes . A series of n i t r opyre ne sta n dard s were spiked i n t o a d i ese l mat ri x and t h e d e t e ction l i m it o f nit r opyrene was deter m ined to be less t h an 1p g . F i g ure 1. S t anda r d. 1) N a p t h ale n e， 2) Ac en ap t hylene 3) Acena pth e ne 4) Fluoren e 5) P h e nanthrene F i gu r e 2. N itro p y r ene (N P ) i d e ntif i ed in fo u r di e se l exhaus t samples us i n g H PLC with f l u o r e s c e n ce detect i on.