第5楼2005/08/15
*如果按照题目 "什么时候适合做 DEPT", 我的答复是 "有机会就检测".
1. 一般核磁谱图的检测, 氢谱不应只是用于最终产品的检测, 而是像 TLC 一样经常性的检测, 用以了解反应进程与粗产品的情况. 至于碳谱或 DEPT 谱图, 则比较像 IR, Mass 一样做为数据的补齐, 得到纯品以后抽空检测获得数据资料.
2. 检测 DEPT 不难, 时间和碳谱差不多. 目前一般学生检测碳谱大约花 15 分钟 (样品浓些), DEPT 也因此 15 分钟便可得到非常满意的信息. DEPT 准确的各级碳的显示, 可以增加自己对于所合成化合物的信心与兴趣.
第6楼2005/08/18
[Selective long-range DEPT NMR technique and its application to structural study of glycoside]
This paper reports a new NMR technique: selective long-range DEPT. The result obtained showed that the technique can be used to assign NMR signal of 13C, 15N and 31P nuclei, and connect the spin systems separated by quaternary carbon or heteroatom. This paper deals mainly with applying the technique for determining the glycosidation position in aglycone moiety and sequence of sugar moiety in a natural glycoside.
Yao Xue Xue Bao. 1992;27(2):107-12.