本人正在做戴安离子色谱的期间核查，请问如何在变色龙软件上获得基线噪声和漂移的值？

应助达人

直接采集基线，然后放大看基线噪音和漂移

Monitor Baseline,然后看Total Signal,漂移值？有这个参数吗，俺是个新手。

我的经验是：
1："FS"的值=进样体积×离子浓度=100.例如Cl-浓度为1.0mg/L，则要求进样体积为100ul。
2：漂移值和噪声，按计量检定标准823-1993，里面有判定和说明的。就是保存至少30min的基线。恕不多言。

这些东西在变色龙的帮助里都有详细说明的，给你拷贝出来看看


基线噪声Signal Noise

The Signal Noise parameter calculates the signal noise within a given time range of the chromatogram. The parameter is used for two main purposes:

The default calculation returns a value that is used internally as the Sensitivity during integration (peak detection).

The signal noise can also be determined for a user-defined time range of the chromatogram, typically for calculating the Signal-to-Noise Ratio.

Signal noise calculation in Chromeleon is based on the ASTM method: After fitting a regression line (using the method of least squares) to all the data points in the time range, Chromeleon determines the maximum distance of the data points above and below the line. (When calculating the regression line, all data points are weighted with their corresponding step unless the step is equidistant.) The signal noise equals the sum of the maximum distances above and below the line.

Determining the Signal Noise for Internal Use (Default)

When no parameters are provided, Chromeleon calculates the signal noise in the first and last 10 seconds of the chromatogram. These values are compared to an internal value. The lowest value is returned as signal noise. If the sensitivity is not explicitly set on the Detection tab of the QNT Editor, this value is then used as the sensitivity for integration (peak detection).

Caution:

To calculate quantification and detection limits via the signal-to-noise ratio, always use the signal noise with a user-defined time range (see below). If you use the default calculation, the quantification and detection limits might not be calculated correctly.

Signal Noise in User-defined Time Range (Parameter Input)

You can also determine the signal noise for a specific time range of the chromatogram. For example, in the Integration view, specify this time range in the Report Column Properties dialog box (after double-clicking the column to edit) or in the Add Report Column dialog box by selecting Add Column on the Table menu. Select Chromatogram in the Categories list, and then select Signal Noise in the Variables list. Click Parameter to open the Parameter Input for 'Signal Noise' dialog box. Select the Specific Range check box and enter a range.

基线漂移Drift

A signal change over time, e.g., caused by a temperature change in the laboratory, gradient elution, or detector drift, is referred to as drift.

To show the drift in Chromeleon: Add a new report variable in the report or in the Printer Layout. Select Insert Chromeleon Report Variable or Insert Column on the context menu to open the appropriate dialog box. In the dialog box, select Chromatogram from the Categories list and Signal Value from the Variables list.

Click Parameter to open the Parameter Input for 'Signal Value' dialog box and select Drift. Select the Restrict Range from check box and determine the range for which the drift is calculated.

To compute the drift, a regression line is drawn through all data points. The slope of the regression line is the calculated drift. Therefore, to compute the drift, always select a baseline range in which no peaks occur.

Note:

Unless the step is equidistant, all data points are weighted with their corresponding steps to determine the regression line.

The drift is always indicated in [Signal]/min.

做个效果图给你看看。

[quote]原文由 啊啊啊(zwql) 发表:做个效果图给你看看。

想请教一下噪音值和基线漂移的那一列是怎么设置的？我的表格只显示NO. Time Peak Name等

上面的说明里不是有吗。简单的说，你用鼠标双击NO. Time等这一行中的任意一个，然后就选吧。如果想新加一个表，就点鼠标右键，选Insert，再选Chromeleon Report Table，后面的慢慢选吧。

谢谢楼上的各位专家，受益匪浅！