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【求助】求助,透射电镜的底片冲洗。

透射电镜(TEM)

  • 悬赏金额:15积分状态:已解决
  • 弱弱的问一句,冲洗底片的步骤是什么?
    我记得在大学的时候上课学的是
    水洗,显影,水洗,定影,水洗,晾干。
    但是现在我不确定了。
    我想问问,在显影和定影中间的那步水洗是不是必须的步骤?为什么?
    还有,显影过度的底片是不是还有救?谢谢大家的帮助。

sian 2006/12/20

[quote]原文由 [B]sian[/B] 发表: 我把师傅发给我的洗底片方法上传了。你看看有没用,我都是这样做的,效果很好[em01][/quote] 好像没有传上,我复制,粘贴到下面吧 Developing of TEM Films 1. Preparation of Stock solution of D19 developer: Dissolve D19 in water according to the manufacturer's instructions 2. Development of KODAK film 4429: Dilute stock solution of D19 developer in water as follows: 1 part of D19 + 2 parts of water Before use, check the temperature of D19 developer . It should be around 20 degrees centigrade. 3. Developing time: 2.5 minutes * with brief agitation every 30 seconds 4. Washing : Wash in diluted acetic acid solution ( 1:20 dilution) for 30 seconds 5. Fixing: Prepare Fixer solution according to the manufacturer's instructions. Fix in the diluted solution for 2-5 minutes 6. Wash in running water for 20 minutes 7. Dry the film (*) The film development time depends on the emulsion setting of your microscope. In our case, we use 2.5 minutes. [img]https://www.instrument.com.cn/bbs/images/affix.gif[/img][url=http://www.instrument.com.cn/bbs/download.asp?ID=35883]Developing of TEM films[/url]

怪味陈皮 2006/12/20

简单的说就是显影-水洗-定影-水洗-晾干,显影和定影之间的水洗是为了洗掉底片上的显影液。 试一下用Photoshop能不能救活显影过度的底片。

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  • sian

    第1楼2006/12/20

    我把师傅发给我的洗底片方法上传了。你看看有没用,我都是这样做的,效果很好

0
    +关注 私聊
  • sian

    第2楼2006/12/20

    好像没有传上,我复制,粘贴到下面吧

    Developing of TEM Films

    1. Preparation of Stock solution of D19 developer:

    Dissolve D19 in water according to the manufacturer's instructions

    2. Development of KODAK film 4429:

    Dilute stock solution of D19 developer in water as follows:
    1 part of D19 + 2 parts of water

    Before use, check the temperature of D19 developer . It should be
    around 20 degrees centigrade.

    3. Developing time:

    2.5 minutes * with brief agitation every 30 seconds

    4. Washing :

    Wash in diluted acetic acid solution ( 1:20 dilution) for 30 seconds


    5. Fixing:

    Prepare Fixer solution according to the manufacturer's instructions.
    Fix in the diluted solution for 2-5 minutes

    6. Wash in running water for 20 minutes

    7. Dry the film

    (*) The film development time depends on the emulsion setting of your
    microscope. In our case, we use 2.5 minutes.


    Developing of TEM films

    sian 发表:我把师傅发给我的洗底片方法上传了。你看看有没用,我都是这样做的,效果很好

0
    +关注 私聊
  • 怪味陈皮

    第5楼2006/12/20

    简单的说就是显影-水洗-定影-水洗-晾干,显影和定影之间的水洗是为了洗掉底片上的显影液。
    试一下用Photoshop能不能救活显影过度的底片。

0
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