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吸收光谱与发射光谱的关系

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  • 编辑部回信的意见如下,请教大虾这是什么意思呢?
    For the free compound, absorption bands are present at 325 and 425 nm. It is stated that fluorescence band appears at 390 when excited to 355. How can be the fluorescence band (at 390) shifted to the blue regarding an absorption band located to 425?. I think that an excitation spectra ad different emission wavelengths could shed light in this apparent inconsistency.
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  • cfgong

    第1楼2019/12/22

    物质有两个吸收峰(325 and 425 nm),而你的荧光激发峰为355, 发射峰为390。 所以425nm处的吸收不是这个荧光峰(ex355 em390)导致。编辑的意思就是让你确认一下425处的吸收是否是这个物质的另一个荧光峰所致,所以让你在其他发射峰再扫一下物质的激发峰(an excitation spectra at different emission wavelengths).

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