null检测 | Characterization of stability, aggregation, and equilibrium properties of modified natural products; The case of carboxymethylated chitosans-美国布鲁克海文仪器公司

方案摘要

方案下载

应用领域
检测样本
检测项目
参考标准

Unstable biopolymer solutions inevitably lead to mis-characterization of macromolecular properties and irreproducible results. Even under stable or quasi-stable conditions, persistent aggregates can hamper reliable characterization, especially using light scattering methods. Various pitfalls in characterizing biopolymers were worked through, including determination of solution stability zones, dissolution kinetics, estimation of fraction of aggregate populations, and the relationship between batch and fractionation methods. Chitosans, polyampholytic biopolymers with isoelectric point around pH=6.0, with varying degrees of carboxymethylation were studied. Instability was determined vs. pH and ionic strength using a high throughput screening method, simultaneous multiple sample light scattering (SMSLS). With stable solution conditions determined, equilibrium batch and multi-detector GPC characterization of molecular weight, intrinsic viscosity, and polyelectrolyte properties was made. Finally, a first attempt at continuous online monitoring of the modification reaction itself was made and compared to FTIR analysis of carboxymethylation on discrete aliquots. Given the range of possible characterization problems, multiple approaches with independent instruments may be required for reliable natural product characterization. Online monitoring of modification reactions may lead to rapid advances in understanding and preparation of natural products.

方案下载

上一篇 NanoBrook产品应用-12-ZetaPALS

下一篇 Coupling of near infrared spectroscopy to automatic continuous online monitoring of polymerization reactions

文献贡献者

美国布鲁克海文仪器公司

金牌会员丨第24年

查看全部资料