方案摘要
方案下载| 应用领域 | 食品/农产品 |
| 检测样本 | 蜜饯 |
| 检测项目 | |
| 参考标准 | / |
Abstract This application note describes the key strategies for pesticide analysis with gas chromatography/triple quadrupole mass spectrometry (GC/TQ) using hydrogen as the carrier gas while maintaining sensitivity to meet maximum residue limits (MRLs). The key aspects addressed in this work include the recommended column configuration, the optimized injection conditions, and the appropriate choice of the mass spectrometer (MS) electron ionization (EI) source hardware developed for use with hydrogen carrier gas. The 20 m × 20 m (0.18 mm × 0.18 μm) Agilent HP-5ms UI midcolumn backflush configuration allowed for maintaining the same retention times as with helium, leading to time savings associated with method translation. The resulting chromatographic resolution achieved under the optimal conditions with hydrogen surpassed that with helium. The optimized injection conditions included solvent vent mode, a 2 mm dimpled liner, and the use of analyte protectants. The analyte response with hydrogen was enhanced on average 10-fold when using the optimized conditions compared to using hydrogen carrier gas with the injection conditions, commonly used with helium. Both the Agilent HydroInert and the Agilent High Efficiency Source (HES) resulted in nearly identical spectra bserved with hydrogen and helium, which allowed using the same multiple reaction monitoring (MRM) transitions and collision energies as with helium. The ability to use the same MRMs, collision energies, and retention times greatly simplified the transition from helium to hydrogen.
The resulting method allowed for quantitation of over 90% of the 203 target pesticides at or below the default MRL of 10 parts per billion (ppb) in the pigmented spinach matrix with both the HydroInert and the HES sources. The method detection limits (MDLs) for compounds susceptible to reactions with hydrogen, and hence, presenting the biggest challenge to the analysis with hydrogen, were in the sub-ppb range, with the HES enabling higher sensitivity and lower MDLs. The calibration performance was demonstrated over a broad range of concentrations, meeting the SANTE/11312/2021 guidelines. The relative standard error (RSE) for over 94% of 203 targets was below 20%. Even the compounds most prone to reacting with hydrogen, such as tecnazene, could be accurately quantitated over the ranges of 0.5 to 5,000 ppb and 0.1 to 1,000 ppb with the HydroInert and HES sources, respectively. Finally, simultaneous dynamic MRM and full scan data acquisition mode was demonstrated for accurate quantitation and reliable compound identification. The identification was based on spectral
matching with the Agilent 8890/7000E and the Agilent 8890/7010C GC/TQ systems using hydrogen carrier gas.
文献贡献者
单克隆抗体的高分离度、高通量体积 排阻色谱分析
采用液相色谱-四极杆串联飞行时间高分辨质谱分析锂电池中的碳酸酯有机溶剂组分
使用 Agilent 5800 ICP-OES 测定固态 电解质锂镧锆钽氧 (LLZTO) 中的 主量元素
相关产品
CrossLab企业资产管理服务
Agilent GC 8890 气相色谱系统
Agilent Cary 3500 紫外可见分光光度计
Agilent Resolve 手持拉曼系统
Agilent TRS100 激光拉曼系统
Agilent 1290 Infinity II 二维液相色谱解决方案
Agilent 1290 Infinity II 液相色谱系统
Agilent 1260 Infinity II 液相色谱系统
Agilent 7900 电感耦合等离子体质谱仪
Agilent Bravo 自动液体处理平台
Agilent 7010C 三重四极杆气质联用系统
Agilent 7000E 三重四极杆气质联用系统
Agilent 5977C GC/MSD 单四极杆气质联用仪
Agilent 1290 Infinity II 高通量系统
Agilent 1290 Infinity II 方法转移系统
关注
拨打电话
留言咨询
