方案摘要
方案下载应用领域 | |
检测样本 | |
检测项目 | |
参考标准 |
The effect of glutaraldehyde on the conformational properties of Kluyveromyces lactis β-galactosidase was determined. The optimum substrate of the enzyme interchanged from lactose to sucrose when it reacted with a certain concentration of glutaraldehyde. Its mechanism was studied by detecting the changes in fluorescence absorption. This is the first discovery that β-galactosidase can be converted to another kind of enzyme which can catalyze sucrose to produce glucose rather than lactose. On the basis of this phenomenon, an amperometric sucrose biosensor using β-galactosidase modified by glutaraldehyde as the immobilized enzyme and poly-β-cyclodextrin as the carrier was prepared. The response characteristic of the sucrose biosensor was also studied. Under optimum conditions, the current response had a linear relationship with the sucrose concentration in the range of 0–2 g/dL with a correlation coefficient of 0.9996 and a response time of < 30 s. The sucrose biosensor exhibited excellent diffusion and stability, and retained its sensitivity after 7 d of continuous use. This sucrose biosensor is expected to provide an effective and economical alternative for the quick and sensitive detection of sucrose in the food and beverage industries in the future.
10个不同品牌食盐的味觉差异分析
日本INSENT电子舌分析奶制品的味觉特征
质构仪技术应用于微针强度的测试和分析
相关产品
关注
拨打电话
留言咨询