脂质覆盖疏水纳米粒子通过磷脂双层自发迁移的直接证明

2017/02/17   下载量： 1 次

方案摘要

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应用领域 生物产业
检测样本 其他
检测项目
参考标准 暂无

采用LaVision公司的PIV系统，配置使用Imager Pro X 型CCD相机，测量脂质覆盖疏水纳米粒子通过磷脂双层自发迁移的速度矢量场

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Hydrophobic nanoparticles introduced into living systemsmay lead to increased toxicity, can activate immune cells, or
can be used as nanocarriers for drug or gene delivery. It is generally accepted that small hydrophobic nanoparticles are
blocked by lipid bilayers and accumulate in the bilayer core, whereas big nanoparticles can only penetrate cells
through slow energy-dependent processes, such as endocytosis, lastingminutes. In contrast to expectations, we demonstrate
that lipid-covered hydrophobic nanoparticles may translocate through lipid membranes by direct penetration
within milliseconds. We identified the threshold size for translocation: nanoparticles with diameters smaller
than 5 nm stay trapped in the bilayer, whereas those with diameters larger than 5 nm insert into the bilayer, opening
pores in the bilayer. The direct proof of this size-dependent translocation was provided by an in situ observation of a
single event of a nanoparticle quitting the bilayer. This was achieved with a specially designed microfluidic device
combining optical fluorescence microscopy with simultaneous electrophysiological measurements. A quantitative
analysis of the kinetic pathway of a single nanoparticle translocation event demonstrated that the translocation is
irreversible and that the nanoparticle can translocate only once. This newly discovered one-way translocation mechanism
provides numerous opportunities for biotechnological applications, ranging from targeted biomaterial elimination
and/or delivery to precise and controlled trapping of nanoparticles.

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北京欧兰科技发展有限公司
金牌会员 丨 第17年
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