其他null检测 | 门宽尺度对基于寿命的福斯特共振能量转移(FRET)参数的估计-北京欧兰科技发展有限公司

方案摘要

方案下载

应用领域	生物产业
检测样本	其他
检测项目
参考标准	暂无

采用德国LaVision公司独特的皮秒门宽增强型CCD相机对鼠抗体样本进行了增强器快门门宽尺度对基于寿命的福斯特共振能量转移(FRET)参数的估计的研究。

方案下载

方案详情

Förster Resonance Energy Transfer (FRET) enables the observation of
interactions at the nanoscale level through the use of fluorescence optical imaging
techniques. In FRET, fluorescence lifetime imaging can be used to quantify the fluorescence
lifetime changes of the donor molecule, which are associated with proximity between
acceptor and donor molecules. Among the FRET parameters derived from fluorescence
lifetime imaging, the percentage of donor that interacts with the acceptor (in proximity) can
be estimated via model-based fitting. However, estimation of the lifetime parameters can be
affected by the acquisition parameters such as the temporal characteristics of the imaging
system. Herein, we investigate the effect of various gate widths on the accuracy of estimation
of FRET parameters with focus on the near-infrared spectral window. Experiments were
performed in silico, in vitro, and in vivo with gate width sizes ranging from 300 ps to 1000 ps in
intervals of 100 ps. For all cases, the FRET parameters were retrieved accurately and the
imaging acquisition time was decreased three-fold. These results indicate that increasing the
gate width up to 1000 ps still allows for accurate quantification of FRET interactions even
in the case of short lifetimes such as those encountered with near-infrared FRET pairs.

上一篇在一个双稳湍流涡旋火焰中，对间歇性动态的时间-频率定位

下一篇球形气泡高斯尾流区域气体-液体扩散系数

文献贡献者

北京欧兰科技发展有限公司

金牌会员丨第17年

查看全部资料