其他理化分析检测 | 表面分析和计算模拟技术相结合研究固定化蛋白质分子的取向效应-北京欧兰科技发展有限公司

方案摘要

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应用领域	石油/化工
检测样本	其他
检测项目	理化分析
参考标准	暂无

采用立陶宛Ekspla公司的和频光谱测量系统（SFG），结合计算模拟分析技术，对固定化蛋白质分子的取向效应进行了实验测量和理论模拟研究

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配置单

Ekspla SFG 表面和频光谱分析系统
型号：SFG

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方案详情

Controlling how proteins are immobilized (e.g. controlling their orientation and conformation) is essential for developing and optimizing the performance of in vitro protein-binding devices, such as enzyme-linked immunosorbent assays. The objective of this work is to develop new methodologies to study proteins and complex mixtures of proteins immobilized onto surfaces. The focus of this study was to control and characterize the orientation of protein G B1, an IgG antibody-binding domain of protein G, on well-defined surfaces as well as measure the effect of protein G B1 orientation on IgG antibody binding using a variety of surface analytical and computational techniques.
The surface sensitivity of time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to distinguish between different proteins and their orientation by monitoring the changes in intensity of characteristic amino acid mass fragments. Amino acids distributed asymmetrically were used to calculate peak intensity ratios from ToF-SIMS data to determine the orientation of five different cysteine mutants of protein G B1 covalently attached to a maleimide surface.

上一篇在一个双稳湍流涡旋火焰中，对间歇性动态的时间-频率定位

下一篇采用等离子体制动器主动控制0.9马赫喷嘴降低噪音

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北京欧兰科技发展有限公司

金牌会员丨第17年

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