其他可靠性能检测 | 用偏振分辨振动和频光谱（SFG-VS）研究寡核苷酸对层状阳离子脂质双层膜的腹层吸附-北京欧兰科技发展有限公司

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应用领域	石油/化工
检测样本	其他
检测项目	可靠性能
参考标准	暂无

应用立陶宛Ekspla公司的偏振分辨振动和频光谱测量系统（SFG-VS）对寡核苷酸对层状阳离子脂质双层膜的腹层吸附过程进行了实验研究和理论分析。

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Ekspla SFG 表面和频光谱分析系统
型号：SFG

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方案详情

The molecular interaction between the oligonucleotides and lipid membranes is the key to the functions of virus, aptamer, and various oligonucleotidebased materials. In this study, the conformational changes of oligonucleotides (dT25) on lamellar cationic 1,2-dimyristoyl-3-trimethylammonium-propane (DMTAP) bilayer were investigated by polarization-resolved sum frequency generation vibrational spectroscopy (SFG-VS) in situ. The SFG-VS spectra within different wavenumber ranges were analyzed to give conformation details of thymine groups, phosphate groups, and OD/OH groups and to provide a comprehensive and fundamental
understanding of the oligonucleotide adsorption on a model bilayer. It is shown that the adsorption of dT25 on DMTAP bilayer reaches maximum at CdT ≈ 500 nM. And the conformation of dT25 molecules change significantly when surface charge of DMTAP bilayer reaches the point of zero charge (PZC) at CdT ≈ 100 nM. Combined spectroscopic evidences also indicate that the formation of electric double layer at the DMTAP/dT25 surface follows the Gouy−Chapman−Stern model. The analysis results also show that the symmetric PO2
− stretching mode of oligonucleotide molecules can serve as a sensitive vibration molecular
probe for quantifying the oligonucleotide/lipid charge ratio and determine the point of zero charge (PZC) of lipid bilayer surface,which may help researchers to control the layer-by-layer assembly of oligonucleotide−lipid complexes and to improve the efficiency genetic therapy against cancer and viral infections.

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北京欧兰科技发展有限公司

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