重组大鼠白介素-2(Rat IL-2)|5μg/20μg/1mg|CYT-382-上海希美化学有限公司

产品详情

IL 2 Rat
Interleukin-2 Rat Recombinant
Catalog No. CYT-382
Synonyms:
T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Introduction:
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Description:
Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 17kDa.
The IL-2 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein (1mg/ml) was lyophilized without any additives.

Solubility:
It is recommended to reconstitute the lyophilized Interleukin 2 in sterile 100mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.

Biological Activity:
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.1-0.4 ng/ml.

Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.535 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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重组大鼠白介素-2(Rat IL-2)由上海希美化学有限公司为您提供，货号CYT-382，规格：5μg/20μg/1mg，CAS号：，如您想了解更多关于重组大鼠白介素-2(Rat IL-2)价格、重组大鼠白介素-2(Rat IL-2)结构式、批发、用途等信息，欢迎咨询。除供应重组大鼠白介素-2(Rat IL-2)外，还可为您提供人白介素2可溶性受体α链(IL-2sRα/CD25)ELISA Kit、重组人白介素-2(Human IL-2)等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

供货周期：	一周
品牌：	Prospect Biosystems
规格：	5μg/20μg/1mg
货号：	CYT-382
CAS号：