AQP7抗体，低价销售

SANTA CRUZ BIOTECHNOLOGY, INC.
AQP7抗体，低价销售 (D-12): sc-376407
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 BACKGROUND
Aquaporins (AQPs) are a large family of integral membrane water transport
channel proteins that facilitate the transport of water through the cell membrane.
This function is conserved in animals, plants and bacteria. Many isoforms
of aquaporin have been identified in mammals, designated AQP0
through AQP10. Aquaporins are widely distributed and it is not uncommon
for more than one type of AQP to be present in the same cell. Although most
aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the
two AQP10 transcripts are also permeable to urea and glycerol. Aquaporins
are involved in renal water absorption, generation of pulmonary secretions,
lacrimation, and the secretion and reabsorption of cerebrospinal fluid and
aqueous humor. Human AQP7 is a 342 amino acid protein that facilitates
water, glycerol and urea transport, and is predominately expressed in adipose
tissue.
REFERENCES
1. Ma, T., et al. 1996. cDNA cloning and gene structure of a novel water
channel expressed exclusively in human kidney: evidence for a gene cluster
of aquaporins at chromosome locus 12q13. Genomics 35: 543-550.
2. Kuriyama, H., et al. 1997. Molecular cloning and expression of a novel
human aquaporin from adipose tissue with glycerol permeability. Biochem.
Biophys. Res. Commun. 241: 53-58.
3. Echevarria, M., et al. 1998. Aquaporins. J. Physiol. Biochem. 54: 107-118.
4. Ishibashi, K., et al. 1998. Molecular characterization of human Aquaporin-
7 gene and its chromosomal mapping. Biochim. 1399: 62-66.
5. Beitz, E., et al. 1999. The mammalian aquaporin water channel family: A
promising new drug target. Curr. Med. Chem. 6: 457-467.
6. Online Mendelian Inheritance in Man, OMIM?. 1999. Johns Hopkins
University, Baltimore, MD. MIM Number: 602974. World Wide Web URL:
CHROMOSOMAL LOCATION
Genetic locus: Aqp7 (rat) mapping to 5q22.
SOURCE
AQP7 (D-12) is a mouse monoclonal antibody raised against amino acids
169-269 mapping at the C-terminus of AQP7 of rat origin.
PRODUCT
Each vial contains 200 μg IgG2a in 1.0 ml of PBS with < 0.1% sodium azide
and 0.1% gelatin.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
PROTOCOLS
See our web site a our catalog for detailed protocols and
support products.
AQP7抗体，低价销售 (APPLICATIONS
AQP7 (D-12) is recommended for detection of AQP7 of rat origin by Western
Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation
[1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence
(starting dilution 1:50, dilution range 1:50-1:500) and solid
phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Molecular Weight (predicted) of AQP7: 37 kDa.
Molecular Weight (observed) of AQP7: 23-33 kDa.
Positive Controls: Rat testis extract: sc-2400.
RECOMMENDED SECONDARY REAGENTS
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Western Blotting: use goat anti-mouse IgG-HRP: sc-2005
(dilution range: 1:2000-1:32,000) or Cruz Marker? compatible goat antimouse
IgG-HRP: sc-2031 (dilution range: 1:2000-1:5000), Cruz Marker?
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecipitation:
use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml).
3) Immunofluorescence: use goat anti-mouse IgG-FITC: sc-2010 (dilution
range: 1:100-1:400) or goat anti-mouse IgG-TR: sc-2781 (dilution range:
1:100-1:400) with UltraCruz? Mounting Medium: sc-24941.
DATA
RESEARCH USE
For research use only, not for use in diagnostic procedures.
AQP7 (D-12): sc-376407. Western blot analysis of
AQP7 expression in rat testis tissue extract.
90 K –
55 K –
43 K –
34 K –
23 K –
AQP7